荷花玉兰组织培养的研究  

Study on tissue culture of Magnolia grandiflora L.

作　　者：王琪[1] 王喆之[2] 李映丽[3] 

机构地区：[1]石河子大学医学院,石河子832002 [2]陕西师范大学生命科学院 [3]西安交通大学药学院

出　　处：《西北药学杂志》2001年第1期11-13,共3页Northwest Pharmaceutical Journal

摘　　要：采用正交设计法考察了４种植物激素对诱导荷花玉兰愈伤组织产生的影响，筛选出最佳的植物激素配比为：ＭＳ培养基＋２，４－Ｄ（２，４－二氯苯氧乙酸，４ｍｇ／Ｌ）＋６－ＢＡ（６－苄基氨基嘌呤，１ｍｇ／Ｌ）＋ＮＡＡ（ａ－萘乙酸，４ｍｇ／Ｌ）；找到最适外植体为花托和叶芽，对比了不同外植体形成愈伤组织的时间、形态和质地的差异。Effects of 2, 4-D, 6-BA, NAA and IAA on callus inducement of Magnolia grandiflora L. were studied by Lg (34) orthogonal design. The best medium for callus inducement was MS+2,4-D(4mg/L) + 6-BA(1mg/L) +NAA(4mg/L). The aceptable explants sellected were receptacle and leaf bud. The callus differences in theperiod, shape and texture formed by different explants had been compared.

关 键 词：荷花玉兰 愈伤组织培养 植物激素 

分 类 号：S682.310.3[农业科学—观赏园艺]