运用组织工程学原理构建许旺细胞三维培养体系  

Study of development of Schwann cells culture system for three-dimensional modeling with tissue engineering

作　　者：黎志明[1] 朱家恺[2] 程钢[1] 朱庆棠[2] 秦允[1] 李志勇[2] 成少安[2] 

机构地区：[1]中山医科大学附属第一医院整形外科,广州市510080 [2]中山医科大学附属第一医院骨科显微外科医学部

出　　处：《中华显微外科杂志》2001年第1期33-35,共3页Chinese Journal of Microsurgery

基　　金：国家自然科学基金!重点项目基金 (39830 1 0 0 );国家自然科学基金!面上项目基金 (39870 80 5);广东省自然科学基金!面上项目基金

摘　　要：目的　探讨普通重力和模拟微重力条件下构建许旺细胞三维培养体系的方法。　方法　对聚羟基乙酸细丝支架进行化学改性处理后再以层粘连蛋白进行生物学修饰 ;分别在普通重力和模拟微重力条件下把原代许旺细胞悬液接种在支架材料上 ,观察许旺细胞的粘附生长情况。　结果　两种重力条件下许旺细胞在聚羟基乙酸支架表面都能良好贴壁生长 ,培养 2 d时细胞生长稳定、密度适中 ;模拟微重力条件下许旺细胞在支架表面的分布更均匀。　结论　普通重力和模拟微重力环境都适宜构建许旺细胞的三维培养体系 ,模拟微重力环境可能更为有利。Objective To develop Schwann cells(SC) culture systems for three dimensional modeling under regular gravity and simulated microgravity Methods Primary Schwnn cells were used to seed polyglycolic acid fibers which were chemicalized and then coated with laminin (LN) under regular gravity and simulated microgravity Results Primary SCs attached excellently to the PGA fibers under both regular gravity and simulated microgravity SCs became stable and their density on the scaffolds was suitable 2 days after seeding SCs distributed more uniformly under simulated microgravity Conclusion Both regular gravity and simulated microgravity are suitable to develop Schwann cells culture systems for three dimensional modeling,and the latter may be better

关 键 词：许旺细胞 三维培养 组织工程学 

分 类 号：R318.08[医药卫生—生物医学工程] R329－33[医药卫生—基础医学]