含神经生长因子的羊膜基质管桥接修复神经缺损的实验研究  

An experimental study peripheral nerve regeneration through NGF·HAMM grafts in rats

作　　者：王平[1] 彭学良[2] 刘晋才[2] 

机构地区：[1]中国人民解放军第二五四医院骨科,天津市300142 [2]重庆第三军医大学大坪医院骨科

出　　处：《中华显微外科杂志》2001年第1期42-45,共4页Chinese Journal of Microsurgery

摘　　要：目的　研究含神经生长因子的人羊膜基质微孔管 (NGF· HAMM)、桥接修复周围神经缺损的效果。　方法　分别用含神经生长因子的人羊膜基质微孔管 (NGF· HAMM)、人羊膜基质微孔管 (HAMM)、自体神经移植修复 6 0只 Wistar大鼠坐骨神经 1.2 cm缺损 ;同时将大鼠按手术方法分为3组 ,每组 2 0只。术后进行组织形态学、电生理学、伤肢功能恢复等检测。　结果　近端神经再生轴突生长通过 1.2 cm长的 NGF· HAMM移植体 ,重新支配终末骨骼肌 ,其再生神经纤维生长速度、数量明显快 (多 )于单纯 HAMM移植体 ,术后 3个月时坐骨神经功能恢复良好。　结论　 NGF和 HAMM联合使用可促进轴突再生及其髓鞘化 ,对损伤神经及伤肢的功能恢复有明显协同促进作用 ;NGF·HAMM是一种很有希望的神经移植替代材料。Objective To determine the effect of peripheral nerve regeneration through NGF·HAMM (Nerve growth factor and Human Ammion membrane Matrix) grafts Methods A 1 2 cm defect of the left sciatic nerve were made and then repaired randomly by means of autogenous nerve bridging (group N),NGF·HAMM microporous conduit bridging (group NH) or HAMM microporous conduit bridging (group H) in 60 adult Wistar rats There were 20 animals in every group At 1,2 and 3 months post operation,the following items as qualitative histology,quatitative histomorphometry,electrophysiology and functional assessments of end organ reconnection were determined Results At 2 and 3 months post operation,Motor nerve conduction velocity (MNCV) of the nerve repaired with HAMM were lower than that of the nerve repaired with autograft and NGF·HAMM At 3 months post operation,there was no significant difference between NH group and N group in nerve conduction velocity ( P >0 05) At 2 and 3 months post operation,both NGF·HAMM conduit and nerve autograft groups showed more nerve fibers,bigger axon diameter and thicker meylin sheaths than H group Conclution The combination of HAMM and NGF world enhance nerve regeneration and myeliogeny,as well as could cooperatively promote the functional recovery of injured nerves and limbs Our results indicate that the NGF·HAMM microporous conduit is a promising tool for use as a nerve guide tube in peripheral nerve regeneration

关 键 词：神经缺损 神经生长因子 羊膜基质管桥接 修复 实验研究 

分 类 号：R651[医药卫生—外科学]