HPLC法测定雪上一枝蒿中雪上一枝蒿甲素含量的方法改进  

Determination of Bullatine A in Aconitum Brachypodum Diels by HPLC

作　　者：陈晓兰[1] 吴玉梅[1] 林亚平[1] 魏文珍 刘毅 徐剑[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵阳市食品药品检验检测中心,贵州贵阳550003

出　　处：《现代妇女（医学前沿）》2014年第1期49-51,共3页Modern Women

基　　金：国家自然科学基金：基于“治疗窗”的雪上一枝蒿微乳经皮给药系统的构建与评价.课题编号：81260646;贵州省科学技术基金项目,课题编号：黔科合J字[2012]2066号;贵州省中医药管理局项目,课题编号：QZYY2011-58.

摘　　要：目的改进雪上一枝蒿药材中雪上一枝蒿甲素的HPLC含量测定方法，为雪上一枝蒿的进一步研究奠定基础。方法在文献研究的基础上，结合预试验，采用70％乙醇回流提取雪上一枝蒿药材，用甲醇溶解提取物，并以KromasilC18色谱柱（150mm×4．6mm，5μm），乙腈-磷酸盐缓冲液（pH=7．3）（18：82）为流动相，流速为1．0mL·min^-1。检测波长为199nm；柱温为30℃。结果采用本含量测定方法，雪上一枝蒿甲素的线性范围为0．2085-4．170μg（r=0．9999），平均回收率分别为99．6％。结论该方法简化了文献报道的前处理过程，操作简便、准确、重复性良好．可为进一步研究其质量控制方法提供参考。Objective To improve a HPLC method for the determination of the content of Bullatine A in Aconitum Brachypodum, and lay the foundation for Aconitum Brachypodum to further study. Method On the basis of literatures and the test.The medicinal materials were isolated by 70% ethanol and the extract was dissolved by methanol. A Kro- masil C18 column （150 mm×4.6 mm,5 μm） was used and the mobile phase consisted of acetonitrile and phosphate buffer（pH of 7.3） （18：82）. The flow rate was 1.0 mL.min^-1 .The detection wavelength was set at 199 nm, and the column temperature was 30 ℃. A HPLC method for the determination of the content of Bullatine A . Result Use the method for determination,The calibration curve was linear （r=0.999 9） in the range of 0.208 5-4.17 μg for Bullatine A , the average recovery of the method was 99.6%. The content of Bullatine A of the medicinal material is about 4.66 mg·g^-1. Conclusion The method simplifies the pretreatment process reported in the literaptue. The method is simple, accurate with good reproducibility, and may provide the reference for the further study its quality control method.

关 键 词：雪上一枝蒿 雪上一枝蒿甲素 含量测定方法的改进 

分 类 号：R284[医药卫生—中药学]