诱导免疫耐受预防大鼠肾脏移植排斥反应的研究  

Prevention of kidney allograft rejection in rat by immune tolerance inductioninduction

作　　者：苑学礼[1] 谢蜀生[2] 刘家望[2] 史其新[1] 郭应禄[1] 

机构地区：[1]北京大学泌尿外科研究所 [2]北京大学医学部免疫学系

出　　处：《中华泌尿外科杂志》2001年第2期69-71,共3页Chinese Journal of Urology

基　　金：国家自然科学基金项目资助(39830340)

摘　　要：目的　探讨具有临床应用前景的移植耐受诱导方法。　方法　以Lewis大鼠和DA大鼠分别作为肾脏移植的受体和供体 ,采用注射抗淋巴细胞血清 (ALS)、输入供体骨髓细胞 (BMT)和环磷酰胺 (Cp)注射方法进行移植耐受诱导 ,观察肾脏移植物的存活情况及受体对供体细胞抗原免疫应答改变。　结果　经耐受诱导的大鼠肾脏移植物存活时间明显延长 ,7只鼠中 5只移植物存活 73～90d时仍无排斥反应迹象 ,混合淋巴细胞反应及诱导迟发型超敏反应表现为供体特异性降低。　结论　诱导免疫耐受预防肾脏移植排斥反应具有重要的临床意义 ,此方法有一定的临床应用前景。Objective To investigate a clinically perspective way of inducing transplantation tolerance. Methods Kidney transplantation model was established using Lewis (RT1 l) rats as recipients and DA (RT1 a) rats as donor. Before transplantation the recipient rats were conditioned with anti lymphothyte serum (ALS) injected for three times, followed by 10 8 donor bone marrow cells transfusion (BMT) and injection of 100 mg/kg cyclophosphamide (CP), survival time of kidney allografts, mixed lymphocyte reaction (MLR) and delayed type hypersensitivity (DTH) of recipient to donor spleen cells were observed and studied. Results 5 of the 7 kidney allografts survived 73 to 90 days without sign of rejection. The donor specific MLR and DTH were suppressed. Conclusions Induction of transplantation tolerance is a prospective way for the prevention of allograft rejection.

关 键 词：肾 移植 免疫耐受性 移植物排斥 

分 类 号：R699.2[医药卫生—泌尿科学]