HPLC法测定拟南芥中总黄酮含量的研究  

Determination of Total Flavonoids Content in Arabidopsis thaliana by High-Performance Liquid Chromatographic

作　　者：张海军[1,2] 郭丽[1] 王明泽[1] 车野[1] 侯丽[1] 李泽宇[1] 王殿奎[1] 

机构地区：[1]黑龙江省农业科学院大庆分院,黑龙江大庆163316 [2]吉林省种子管理总站,吉林长春130062

出　　处：《黑龙江农业科学》2014年第4期97-100,共4页Heilongjiang Agricultural Sciences

基　　金：教育部新教师基金资助项目(3M2101936604);国家麻类产业技术体系资助项目(NYCYTX-19-S13);黑龙江省农业科技创新工程重点(青年基金)资助项目

摘　　要：为更好地开发和利用拟南芥中总黄酮及相关副产品，研究了检测拟南芥中总黄酮含量的方法。结果表明：以芦丁为标准品，建立了检测拟南芥中总黄酮含量的HPLC法。HPLC法色谱条件：C18色谱柱、35℃柱温、256nm检测波长、35％甲醇：65％水（含0．4％磷酸）流动相、10ML进样量及10mL·min-1流速。标准品芦丁在该色谱条件下，峰型良好，在0．05～1．60mg·mL^-1时峰面积与质量浓度间呈良好线性关系，R2=0．9997，平均加样回收率为99．90％，RSD小于3％（n=6），该方法适用于拟南芥中总黄酮的检测。In order to develop and use the flavonoids and related by-products in Arabidopsis thaliana, the deter- mine method was established for total flavonoids content. The results showed that taking rutin as the standard, high-performance liquid chromatographic method was established, mobile phase consisted on methanol （A）/ 0.4% orthophosphoric acid in distrilled water（B）and each solvent was filtered through a 0.45 μm pore size fil- ter and degassed in an ultrasonic bath before. The analysis of rutin was performed at 35℃ （column oven tem- perature）with 10 mL.min1 flow rate, the injection volume was 10 μL,and solvent A：B of rutin was 35%： 65% ,the chromatogram was detected at 256 nm. The peak area had a good linearity with concentration when R2 ： 0. 999 7, the recoveries of standard spiling was 99.90 % with RSD less than 3 %. So the method was suit- able for determination of total flavonoids in Arabidopsis thaliana.

关 键 词：拟南芥 总黄酮含量 芦丁 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]