超高效液相色谱法同时测定益智中4种抗氧化活性成分  

Simultaneous Quantitation of Four Antioxidant Components in Alpinia oxyphylla Miq. by UPLC

作　　者：石绍淮[1] 刘冰[1] 李欢[1] 刘爱敬[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院、沈阳市中药药效物质基础筛选与评价重点实验室,沈阳110016 [2]沈阳药科大学药学院、中药质量控制关键技术国家地方联合工程实验室,沈阳110016

出　　处：《中国药学杂志》2014年第7期592-595,共4页Chinese Pharmaceutical Journal

基　　金：辽宁省科学技术计划项目资助(2011412004-1);沈阳市科技计划项目(F12-153-9-00)

摘　　要：目的建立同时测定不同产地的益智的不同部位中5-羟甲基糠醛（1），teuhetenoneA（2），杨芽黄素（3）和诺卡酮（4）含量的超高效液相色谱法（UPLC）。方法采用ACQUITYUPLC，HSS33色谱柱（2．1mm×100mm，1．8μm），流动相为乙腈-0．1％的甲酸水溶液梯度洗脱，流速为0．5mL·min-1，检测波长采用255nm。结果化合物1，2，3和4分别在1．223～24．46，2．016～40．32，1．875～37．50和16．78～335．6μg·mL-1线性关系良好，平均回收率分别为99．5％，101．5％，100．9％和101．2％。结论化合物2和4主要分布于种子中，而化合物1和3均分布于种子和果壳中；该方法快速、简单、重复性好，可以为益智的鉴别和质量控制提供依据。OBJECTIVE To establish a UPLC method for simultaneous determination of 5-hydroxymethyl furfural （1）, teu- betenone A （2）, tectochrysin （3）, and nootkatone （4）in Alpinia oxyphylla Miq, and to compare the contents of the four components in different parts of this medicinal materials from different places. METHODS The UPLC method was established on an HSS T3 Col- umn （2. 1 mm × 100 mm, 1.8 μm）. The mobile phase consisted of water containing 0. 1% formic acid and acetonitrile in gradient e- lution mode at the flow rate of 0. 5 mL .min - 1and the detection wavelength was set at 255 nm. RESULTS The standard curves of compounds 1, 2, 3, and 4 showed good linearity in the ranges of 1. 223 -24. 46, 2. 016 -40. 32, 1. 875 -37.50 and 16. 78 -335.6 μg .mL-1 with the corresponding average recoveries of 99. 5% , 101.5% , 100. 9% and 101.2% , respectively. CONCLUSION The method is precise and highly reproducible, which can be used to simultaneously determine the antioxidant components including 5- hydroxymethyl furfural, teuhetenone A, tectochrysin, and nootkatone in Alpinia oxyphyUa Miq; compounds 2 and 4 are mainly extrae- ted from the seeds, while compounds 1 and 3 come from the seeds and putamens equally. There are significant differences among the samples from different production areas.

关 键 词：益智 超高效液相色谱法 5一羟甲基糠醛 teuhetenone A 杨芽黄素 诺卡酮 

分 类 号：R284[医药卫生—中药学]