钴-60伽玛射线诱导淋巴细胞γH2AX表达的研究  

Study on γH2AX expression of human lymphocytes induced by ^(60)Co gamma-rays

作　　者：潘艳[1] 高刚[1] 刘澜涛[1] 阮健磊[1] 刘建香[1] 苏旭[1] 

机构地区：[1]辐射防护与核应急中国疾病预防控制中心重点实验室中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《辐射研究与辐射工艺学报》2014年第2期9-12,共4页Journal of Radiation Research and Radiation Processing

基　　金：卫生行业科研专项(201002009);中国疾病预防控制中心青年科研基金课题(2012A203)资助

摘　　要：采用流式细胞术检测60Coγ-射线照射后γH2AX表达的量效和时程关系,同时探讨紫外线联合60Coγ-射线照射后γH2AX的表达情况。结果表明,不同剂量60Coγ-射线照射后γH2AX表达量与照射剂量之间呈现明显的剂量效应关系。4 Gy 60Coγ-射线照射后,γH2AX的表达在1 h达到峰值,随后逐渐减少。与假照组相比,单纯紫外线照射组γH2AX表达增加,一直稳定维持约6 h左右,随后开始减少。紫外线联合60Coγ-射线照射组与单纯60Coγ-射线照射比较,γH2AX的表达无显著性差异。结果提示采用流式细胞术检测γH2AX的表达估算受照剂量具有可行性,该法有望成为估算辐射剂量的新方法。Flow cytometry analysis method was used to detect the changes of yH2AX protein expression in human peripheral blood lymphocytes. In the dose-effect study, peripheral blood was irradiated by 60Co γ-rays with the dose of 0-6 Gy and cultivated for 1 h. Peripheral blood were cultivated for 0-24 h after 4 Gy 60Co γ-rays irradiation for the time-effect study. Then, the peripheral blood was divided into four treatment groups, i.e. UV irradiation, 60Co γ-rays irradiation, UV plus 60Co γ-rays irradiation and control group to detect the changes of protein expression in different time. The results showed that the yH2AX protein expression was in a dose-dependent form. The expression of yH2AX peaked at 1 h after 4 Gy irradiation and began to decrease quickly. The expression of yH2AX in UV irradiation group increased compared with that of the sham-irradiation control group, and the expression of yH2AX peaked at 6 h. While compared with that of the 60Co γ-rays group, the yH2AX expression of UV combined 60Co γ-rays group was unchanged. The results reveal that the detection of 7H2AX protein expression changes in peripheral blood lymphocyte by flow cytometry analysis is reasonable and may turns be a new way for biological dosimeter.

关 键 词：^60CO γ-射线 7H2AX 紫外线 DNA损伤 生物剂量计 

分 类 号：R811.5[医药卫生—放射医学] Q691.5[医药卫生—临床医学]