电离辐射联合顺铂对人肺癌细胞中BTG3基因表达的影响  

Effects of ionizing radiation and cisplation on the expression of BTG3 in human lung cancer cell lines

作　　者：王利利[1] 涂彧[2] 贺汝燕[1] 秦颂兵[1] 徐晓婷[1] 周菊英[1] 

机构地区：[1]苏州大学附属第一医院放疗科,苏州215006 [2]苏州大学医学部放射医学与防护学院江苏省放射医学与防护重点实验室,苏州215123

出　　处：《辐射研究与辐射工艺学报》2014年第2期13-18,共6页Journal of Radiation Research and Radiation Processing

基　　金：江苏省放射医学与防护重点实验室开放课题(KJS1239)资助

摘　　要：加速器产生的6-MV X-射线一次性照射体外培养的人肺腺癌细胞株A549和SPCA-1,细胞的吸收剂量分别设置为0、1、3、5和8 Gy,细胞照射后24 h及单次接受5 Gy照射后6、12和24 h后取样;X-射线联合顺铂对BTG3表达的影响研究设立单纯照射组(5 Gy)、顺铂处理组(20μmol·L-1顺铂)和联合处理组(5 Gy+20μmol·L-1顺铂),上述实验均以未给予任何处理的细胞为对照组;采用Western blot及RT-PCR法检测受到不同剂量电离辐射及照射后不同时间肿瘤细胞中BTG3表达水平。Western blot与PCR检测结果均显示,A549和SPCA-1细胞在受到不同剂量的X-射线照射后24 h,其BTG3蛋白及mRNA表达量均明显增加,并随电离辐射剂量的增加而增加,与对照组比较有统计学差异(t=5.25-15.75,p<0.05);照射后不同时间检测结果显示,细胞在X-射线照射4 h后BTG3蛋白及mRNA的表达量即开始上升,并持续到照射后24 h,与照射前相比有统计学差异(t=7.52-11.18,p<0.05);联合处理组BTG3的表达量均明显高于单纯照射组、顺铂处理组和对照组(t=7.02-15.86,p<0.05)。电离辐射联合顺铂可以上调肺癌细胞株中BTG3的表达,BTG3基因有可能作为肺癌放化疗的靶基因。Human lung cancer A549 and SPCA- 1 cell lines were irradiated by X-rays at the absorbed doses of 1, 3, 5 and 8 Gy, respectively, generated by a linear accelerator （with the source skin distance of 100 cm and dose rate of 200 cGy＇minX）. The cell molecular samples were subtracted at 6 h, 12 h, and 24 h after 5 Gy ionizing irradiation. For the study of X-rays and cisplatin combination treatment, cells were divided into radiation group （5 Gy X-rays）, cisplatin group （20 gmol-L-1 cisplatin）, and combination group （5 Gy X-rays ＋ 20 pmol-L 1 cisplatin）. Untreated cells were classified as control group. The relative levels of BTG3 mRNA and protein expression in cells were detected by semi-quantitative RT-PCR and Western blot analysis with quantitation of the mRNA/protein bands by densitometry. The expression level of BTG3 mRNA and protein significantly increased with the dose climbed after radiation exposure of human lung cancer A549 and SPCA-1 cell lines （t=-5.25-15.75, p〈0.05）. The expression level in cancer cells significantly increased 4 h after radiation exposure and kept increasing until 24 h later （t=-7.52-11.18, p〈0.05）. Compared with that of the radiation and/or cisplatin treatment alone, more significant increase of BTG3 expression was identified after the combination treatment of X-rays and cisplatin （t=-7.02-15.86, p〈0.05）. Ionizing radiation could up-regulate the expression of BTG3 in human lung cancer cells in both mRNA and protein levels. BTG3 gene may be targeted as a molecular for ionizing radiation and cisplatin treatment in lung cancer.

关 键 词：BTG3 肺癌细胞系 电离辐射 顺铂 

分 类 号：R811.5[医药卫生—放射医学]