氟化物对家蚕耐氟和氟敏感品种肠道中留存产酶菌群落组成和多样性的影响  

Effects of fluoride on the community composition and diversity of the retained enzyme-producing bacteria in the intestine of silkworm (Bombyx mori)

作　　者：李冠楠[1] 夏雪娟[2] 赵欢欢[1] 隆耀航[1] 李姣蓉[1] 朱勇[1] 

机构地区：[1]西南大学生物技术学院,重庆400716 [2]西南大学食品科学学院,重庆400716

出　　处：《昆虫学报》2014年第2期187-193,共7页Acta Entomologica Sinica

基　　金：国家农业科技成果转化资金(2012GB2F100376)

摘　　要：【目的】研究氟化物对家蚕Bombyx mori肠道内留存产酶菌的影响,有助于了解家蚕耐氟和氟敏品种的耐氟力差异。【方法】分别给家蚕耐氟品种T6和氟敏品种734添食NaF溶液浸泡后的新鲜桑叶,至5龄第3天取材。采用筛选培养基筛选产蛋白酶、纤维素酶、脂肪酶、淀粉酶的菌株,并结合16S rDNA系统发育关系,对菌株进行分类鉴定。【结果】家蚕肠道内产消化酶细菌有芽孢杆菌属Bacillus、葡萄球菌属Staphylococcus、肠杆菌属Enterobacter和不动杆菌属Acinetobacter和微小杆菌属Exiguobaterium,其中芽孢杆菌Bacillus sp.、葡萄球菌Staphylococcus sp.和不动杆菌Acinetobacter sp.细菌可同时产4种酶。氟中毒后T6肠道内产酶菌由5种减少到4种,734肠道内产酶菌由2种减少到1种。【结论】家蚕肠道内留存的产酶菌与家蚕自身的耐氟能力相关。[ Aim] Research the influence of fluoride on the retained enzyme-producing bacteria in the intestine of the silkworm, Bombyx mori so as to contribute to understanding the difference of the enduring fluoride between fluoride-resistant and sensitive varieties. [ Methods ] Fluoride-resistant variety T6 and fluoride-sensitive variety 734 of the silkworm were fed with fresh mulberry leaves soaked in NaF solution until the 3rd day of the 5th instar for experiment. Protease-, cellulase-, lipase- and amylase-producing strains were screened on selection medium, and the taxonomic composition of the intestinal bacterial community was identified according to phylogenetic relationship based on 16S rDNA sequences. [ Results ] Genera producing digestive enzymes in the silkworm intestine included Bacillus, Staphylococcus, Enterobacter , Acinetobacter , and Exiguobacterium , among which Bacillus sp., Staphylococcus sp. and Acinetobacter sp. bacteria produced four kinds of enzymes at the same time. The species of enzyme-producing bacteria in the intestine of T6 and 734 varieties reduced from 5 to 4 and 2 to 1, respectively after fluorosis. [ Conclusion ] The retained enzyme-producing bacteria in silkworm intestine are related to the ability of enduring fluoride of silkworm.

关 键 词：家蚕 肠道细菌 产酶菌 氟化物 氟中毒 耐氟品种 

分 类 号：Q965.8[生物学—昆虫学]