不同肠内营养制剂对小鼠肝切除术后肝再生及其结构功能的影响  

Effects of two kinds of enteral nutrition preparations on liver regeneration and function in mice after partial hepatectomy

作　　者：段飞[1] 陈靓[1] 訾雪剑 伏旭[1] 姚辉[1] 伏晓[1] 仇毓东[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肝胆胰外科,江苏省南京市210008

出　　处：《世界华人消化杂志》2014年第9期1193-1200,共8页World Chinese Journal of Digestology

摘　　要：目的:探讨两种不同肠内营养(enteral nutrition,E N)制剂对小鼠肝切除术后肝脏再生及其结构功能的影响.方法:建立小鼠70%肝切除术后口服EN模型,♂ICR小鼠72只随机均分为70%肝切除对照组(术后常规饮食)、EN混悬液TP-MCT组(70%肝切除后选用TP-MCT喂养)、EN混悬液SP组(70%肝切除后选用SP喂养).检测术后1、3、7d肝功能指标,肝再生情况和残肝组织病理学改变及相关炎性因子的情况.结果:与对照组相比,SP组及TP-MCT组术后1、3、7 d外周血中谷丙转氨酶、谷草转氨酶有所降低,差异有统计学意义(P<0.05);与对照组相比,SP组及TP-MCT组术后第1天血清抗炎因子白介素-10(interleukin-10,IL-10)水平有所上升,血清促炎因子IL-6水平有所降低(P<0.05);与对照组相比,HE染色及PCNA显示SP组和TP-MCT组术后肝再生明显,肝脏结构得到恢复,特别是SP组于术后1、3、7 d呈现显著性肝脏再生.结论:小鼠肝切除后予以EN,可以保护术后肝脏结构功能,促进肝再生,应用EN混悬液SP优于TP-MCT.AIM： To investigate the effects of two kinds of enteral nutrition preparations on liver regenera- tion and function in mice after partial hepatec- tomy （PH）. METHODS： A mouse model of oral enteral nu- trition （EN） after 70%PH was used in this study. Seventy-two male ICR mice were randomly di- vided into a PH control group （routine diet after PH）, a TP-MCT group （EN with TP-MCT after PH） and a SP group （EN with Peptisorb after PH）. Serum samples and liver tissue specimens were collected 1, 3 and 7 d after PH. Serum sam- ples were used to measure the levels of alanineaminotransferase （ALT）, aspartate transaminase （AST）, albumin, interleukin-6 （IL-6） and IL-10. The liver tissue specimens were used to measure the proliferation of hepatocytes and the mor- phology of liver tissue by immunohistostaining and HE staining. RESULTS： In comparison with the PH control group, serum levels of ALT and AST on days 1, 3 and 7 decreased significantly in the TP-MCT group and SP group （P 〈 0.05 for all）. Compared with the PH control group, the level of IL-6 on day 1 decreased significantly in the TP-MCT group and SP group, but the level of IL-10 in- creased significantly in the TP-MCT group and SP group （P 〈 0.05 for all）. Liver regeneration was enhanced significantly on days 1, 3 and 7 in the TP-MCT group and SP group, especially in the SP group. CONCLUSION： Enteral nutrition can well pro- tect mouse liver function and promote liver re- generation after PH, with SP （Peptisorb） having a better effect.

关 键 词：肝切除 肠内营养 肠内营养混悬液SP 肠 内营养混悬液TP—MCT 

分 类 号：R657.3[医药卫生—外科学]