益生菌及其联合新城疫疫苗免疫雏鸡局部黏膜组织IL-2mRNA表达的变化  

Change of IL-2 mRNA Expression in local mucosal tissues of Probiotics broilers Vaccinated with nd vaccine

作　　者：于志强[1] 吕晓萍[1] 高雪丽[1] 刘超男[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2014年第2期20-23,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30972162)

摘　　要：以艾维因肉雏鸡为研究对象,应用实时荧光定量PCR方法,通过Ⅱ-2mRNA表达的检测,较全面系统的研究了益生菌及其联合新城疫（ND）疫苗免疫雏鸡后,其十二指肠、回肠、哈德尔氏腺和盲肠扁桃体IL-2 mRNA表达的动态变化.结果发现,雏鸡应用益生菌后,其上述局部黏膜免疫组织IL-2 mRNA表达均不同程度高于对照雏鸡,并于4～7 d差异显著或极显著（P＜0.05或P＜0.01）;益生菌联合ND疫苗免疫雏鸡,上述被检组织的IL-2 mRNA表达虽然高于ND疫苗单独免疫雏鸡,但未见统计学差异（P＞0.05）.表明益生菌不但能够增强机体的免疫功能,而且与疫苗联合应用后,能够提高疫苗的免疫应答.AVIAA broilers were used in this study. The dynamic change of IL-2 mRNA expression in duodenum, ileum, Harder's gland and cecal tonsil of broilers which were fed with probiotics and vaccinated by Newcastle disease(ND) vaccine was detected by RT-PCR. The results showed that the above-mentioned local mucosal immune tissue IL-2 mRNA expression of the broilers fed with probiotics was to some degree higher than that of the control ones. the difference was significant or highly significant(P <0.05 or P <0.01) in 4 to 7 days. The above-mentioned partial mucosal immune tissue IL-2 mRNA expression of the broilers fed with probiotics and vaccinated by ND vaccine was to some degree higher than that of the vaccinated only. But there was no significant difference(P >0.05). These data show that probiotics can enhance the body's immune function,and improve the immune response to the vaccine.

关 键 词：益生菌 新城疫疫苗 白细胞介素-2 MRNA表达 

分 类 号：S852.4[农业科学—基础兽医学]