丹酚酸B对小鼠日本血吸虫病肝纤维化MMP-1、TIMP-1的影响  

Impacts of salvianolic acid B on matrix metalloproteinase-1 and tissue inhibitors of metalloproteinsase-1in mice with schistosomiasis japonicum and hepatic fibrosis

作　　者：郭鄂平[1] 杨树国[1] 张珍[2] 朱明磊[1] 王娅[1] 

机构地区：[1]湖北医药学院寄生虫学教研室,十堰442000 [2]湖北医药学院附属太和医院检验部

出　　处：《陕西医学杂志》2014年第3期259-261,共3页Shaanxi Medical Journal

基　　金：湖北省卫生厅专项科研项目(XF2010-26)

摘　　要：目的:探讨丹酚酸B治疗小鼠血吸虫病肝纤维化,血清MMP-1与TIMP-1的变化及肝组织MMP-2与TIMP-2的基因表达。方法:随机将未感染和感染日本血吸虫尾蚴昆明小鼠分成A^F 6组。即未感染空白对照组(A组)、模型组(B组)、溶剂组(C组)、丹酚酸B治疗低剂量[5mg/(kg·d)]组(D组)、中剂量[10mg/(kg·d)]组(E组)、高剂量[15mg/(kg·d)]组(F组),每组10只。实验结束处死小鼠,留取肝脏组织和血清。用ELISA法检测血清MMP-1及TIMP-1的含量,逆转录-聚合酶链反应法(RT-PCR)检测小鼠肝组织MMP-1mRNA及TIMP-1mRNA的表达。结果:免疫组化检测显示,给予丹酚酸B治疗后,D、E、F组的MMP-1蛋白表达增加,而TIMP-1蛋白表达降低,与模型组比较差异有统计学意义。逆转录PCR检测结果表明,D、E、F组的MMP-l mRNA表达增加,而TIMP-1mRNA表达降低,与模型组比较差异有统计学意义。结论:丹酚酸B具有抗血吸虫性肝纤维化作用,可能通过增加MMP-1表达,抑制TIMP-1表达而促进肝纤维化的逆转。Objective：To study the effects of salvianolic acid B （SAB） ,on serum levels of matrix metallo-proteinase-1 （MMP-1） and tissue inhibitors of metalloproteinsase-1 （TIMP-1） ,along with hepatic expression of MM P-1 and TIMP-1 mRNA in mice with hepatic fibrosis induced by Schistosoma japonicum .Methods ：The mice model of hepatic fibrosis which infected by cercariae of S .japonicum were randomly divided into 6 groups ：Group A as a blank control ,Group B as a model ,Group C as a solvent ,Group D ,E ,F treated with a low dose of 5mg/（kg·d） ,middle dose of 15mg/（kg·d） ,and high dose of 15mg/（kg·d） of SAB ,respectively .Every group had 10 mice .All the mice were killed at the end of the administration .Serum and liver tissues were kept back .Serum levels of MMP-1 and TIM P-1 were detected by ELISA .The hepatic mRNA expression of MMP-1 and TIMP-1 were deter-mined by reverse transcription-polymerase chain reaction （RT-PCR） at the same time .Results ：The significance in-creased the expression of MMP-1 protein and decreased the expression of TIMP-1 protein were observed .RT-PCR results showed that the expression of MM P-l mRNA was enhanced and the expression of TIMP-1 mRNA was de-creased significantly .Conclusion ：SAB has an obviously inhibitory effect on the hepatic fibrosis induced by Schisto-soma japonicum infection .One of the possible mechanisms is to raise the expression of MMP-1 and reduce the ex-pression of TIMP-1 .

关 键 词：@丹酚酸B 日本血吸虫病 寄生虫学 肝纤维化 基质金属蛋白酶-1 代谢 @组织金属蛋白酶抑制因子-1 小鼠 

分 类 号：R383[医药卫生—医学寄生虫学]