鱿鱼墨多肽制备及其诱导DU-145和PC-3细胞早期凋亡研究  

The Squid Ink Peptides Inhibit Growth of DU-145 and PC-3 by Inducing Apoptosis

作　　者：李丽[1] 朱亚珠[1] 胡晓斐[2] 黄芳芳 丁园芳 丁国芳 

机构地区：[1]浙江国际海运职业技术学院,浙江舟山316021 [2]浙江省舟山医院,浙江舟山316021 [3]浙江海洋学院食品与药学学院浙江省海洋生物医用制品重点工程技术研究中心,浙江舟山316022

出　　处：《中国食品学报》2014年第2期35-41,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(81273429);浙江省科技厅重大专项(2010C13009;2011C02003);浙江江省海洋生物技术产业重点科技创新团队(2010R50029);浙江省自然科学基金项目(Y3100129;Y2100805;LY12C20005);浙江省科技计划项目(2011C33014;2011C23034);舟山市科技计划项目(092016)

摘　　要：目的:研究鱿鱼墨多肽酸法提取工艺及不同浓度鱿鱼墨多肽对DU-145和PC-3细胞的增殖抑制和诱导凋亡作用。方法:以料液比、加酸量、酸解时间和酸解温度为因素,以酸解液的氨基氮含量为指标,通过正交试验确定提取鱿鱼墨多肽的酸解条件。采用CCK-8法检测其对DU-145和PC-3的生长抑制作用。采用Annexin V-FITC/PI双标记流式细胞术检测和AO/EB双染法,观察鱿鱼墨多肽对DU-145和PC-3细胞的早期凋亡情况。结果:酸法提取鱿鱼墨多肽最佳组合为0.02 mol/L盐酸、酸提料液比1∶1、酸解时间2 h、酸解温度35℃;鱿鱼墨多肽对DU-145和PC-3细胞有增殖抑制作用,能诱导DU-145和PC-3细胞凋亡。结论:鱿鱼墨多肽能抑制DU-145和PC-3细胞增殖,并诱导其细胞凋亡。Objective： To study the preparation process of Squid ink peptides and its antitumor effect in vitro. Methods： The better acid was selected according to the contents of amino nitrogen of hydrolysates, which were prepared by hydrolyzing Squid ink with I＇/ydroehloric acid. The anti-proliferation activity of Squid ink peptides was detected by CCK-8. Apoptosis in DU-145 and PC-3 after treatment with Squid ink peptides was observed by AO/EB staining. In addition, its early apoptosis in DU-145 and PC-3 was detected by AnnexinV-FITC and PI double staining. Results： The result of CCK-8 showed that the Squid ink peptides （5-30 mg/mL） inhibited DU-145 and PC-3 cells growth in a dose-dependent manner. The early stage of apoptosis rate was increased by inducing with the Squid ink peptides. Typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining, Conclusion： The Squid ink peptides inhibit growth of Prostate Cancer Cell Lines by inducing apoptosis.

关 键 词：鱿鱼墨多肽 DU-145细胞株 PC-3细胞株 细胞凋亡 

分 类 号：R151[医药卫生—营养与食品卫生学]