慢性阻塞性肺疾病大鼠气道炎症与白细胞介素10的相关性研究  

The role of Interleukin-10 in Airway Inflammation in COPD Rats

作　　者：汪新龙[1] 刘朝晖[1] 梁志科[1] 范伟[1] 马碧蔓[1] 关丽君[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院,广东广州510180

出　　处：《中国呼吸与危重监护杂志》2014年第2期142-145,共4页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：广东省自然科学基金项目(编号:101510060010000 12);广州市卫生局资助项目(编号:30504382207);广东省医学科研基金(编号:A2013495)

摘　　要：目的研究慢性阻塞性肺疾病(简称慢阻肺)大鼠模型中白细胞介素10(IL-10)、肿瘤坏死因子ɑ(TNF-α)、干扰素-γ(IFN-γ)在血清和肺组织中的表达,探讨IL-10在慢阻肺气道炎症中的作用及调控机制。方法成年雄性SD大鼠45只,分为模型组30只,对照组15只,采用反复熏香烟+气管内注入脂多糖法建立慢阻肺大鼠模型。ELISA法检测大鼠血清、肺组织中IL-10、TNF-ɑ和IFN-γ的表达。结果模型组血清和肺组织内TNF-α较对照组显著升高,IFN-γ、IL-10[血清(44.68±8.67)ng/L比(75.96±10.59)ng/L,肺组织(64.55±9.03)ng/L比(94.06±8.71)ng/L,P均<0.01]表达均下降。IL-10和TNF-ɑ水平呈负相关(血清r=-0.67,肺组织r=-0.80,P<0.01)。IL-10和IFN-γ水平呈正相关(血清r=0.64,肺组织r=0.72,P<0.01)。结论 IL-10的下调在慢阻肺大鼠气道炎症中起重要的调控作用。Objective To investigate the expressions of IL-10, tumor necrosis factor-α （TNF-ct） and interferon-γ （IFN-γ） in serum and lung tissue of COPD rats in order to elucidate the potential mechanism of airway inflammation. Methods Forty-five healthy adult male SD rats were randomly divided into a COPD model group （ n = 30） and a normal control group （ n = 15 ）. The COPD rat model was established by intratracheal instillation of lipopolysaccharide （LPS） and exposure to cigarette smoke for 28 days. The concentrations of IL-IO, TNF-α and IFN-γ in serum and lung tissue were measured by ELISA. Results TNF-α level of serum and lung tissue in the COPD model group increased significantly compared with the control group （ P 〈 O. 05 ）, while the levels of IFN-γ and IL-10 decreased significantly [ serum ： （44.68±8.67） ng/L vs. （75.96± 10.59） ng/L;lung tissue： （64.55±9.03） ng/L vs. （94.06 ± 8.71 ） ng/L,P 〈0. O1 ]. The level of IL-10 in serum and lung tissue was negatively correlated with TNF-α （serum：r = -0. 67, lung tissue： r = -0. 80, P 〈 0. 01 ）. The level of IL-IO in serum and lung tissue was positively correlated with IFN-γ （ serum： r = 0. 64, lung tissue ：r = 0. 72, P 〈 0. 01 ）. The level of IL-10 in serum and lung tissue was negatively correlated with the percentage of neutrophils （ serum： r = - 0. 70, lung tissue ： r = - 0. 67, P 〈 0. 01 ）. Conclusion In COPD rats, down regulation of IL-10 plays an important role in regulation of airway inflammation.

关 键 词：慢性阻塞性肺疾病 气道炎症 白细胞介素10 肿瘤坏死因子Α Γ-干扰素 

分 类 号：R563.9[医药卫生—呼吸系统]