高效液相色谱法测定复方矮地茶止咳合剂中的槲皮素和山奈酚含量  

HPLC determination of quercetin and kaempferol in Fufang Aidicha Zhike mixture

作　　者：易长英 

机构地区：[1]怀化市中医院,湖南怀化418000

出　　处：《中南药学》2014年第1期66-69,共4页Central South Pharmacy

摘　　要：目的建立高效液相色谱法测定复方矮地茶止咳合剂中槲皮素和山奈酚的含量。方法采用SUPELCO Discovery C18（4．6mm×250nm，5μm）色谱柱，流动相：甲醇-0．4％磷酸溶液（50：50），流速：1．0mL·min^-1，检测波长：370nm，柱温：40℃，进样量：10μL。结果槲皮素和山奈酚分别在6．484-155．6μg·mL^-1、5．708～137．0μg·mL^-1与峰面积线性关系良好，低、中、高3个剂量组的平均回收率分别为93．6％、97．2％、98．2％与95．2％、94．9％、100．2％。结论所建立的方法准确可靠、灵敏度高、专属性强，可有效控制复方矮地茶止咳合剂的质量。Objective To establish a method for the determination of quercetin and kaempferol in Fufang Aidicha Zhike mixture by HPLC. Methods SUPELCO Discovery C18 （4.6 mm×250nm,5μm） was used with methanol-0.4% phosphoric acid （50 ： 50） as the mobile phase. The flow rate was 1.0 mL·min^-1, the detection wavelength was 370 nm, the column temperature was 40 ℃, and the sample amount was 10 μL. Results The linear ranges of quercetin and kaempferol were 6.484 - 155.6 μg·mL^-1 and 5.708 - 137.0 μg·mL^-1, respectively. The average recoveries were 93.6%, 97.2%, 98.2% and 95.2%, 94.9%, 100.2%, respectively. Conclusion The method is reliable, accurate and spe- cific with a good reproductivity and which be used to control the quality ofFufang Aidicha Zhike mixture.

关 键 词：复方矮地茶止咳合剂 高效液相色谱法 槲皮素 山奈酚 

分 类 号：R917[医药卫生—药物分析学]