高效液相色谱法测定白术中白术内酯Ⅰ、Ⅱ和Ⅲ的含量  

Simultaneous determination of atractylenolide Ⅰ, Ⅱ, and Ⅲ in Atractylodes macrocephala Koidz. by HPLC

作　　者：李羾 何丹[2] 杨常成[2] 

机构地区：[1]上海市电力医院药剂科,上海200050 [2]中国人民解放军第163医院药剂科,长沙410003

出　　处：《中南药学》2014年第1期70-73,共4页Central South Pharmacy

摘　　要：目的建立白术中白术内酯Ⅰ、Ⅱ及Ⅲ的含量测定方法。方法采用高效液相色谱法，资生堂CAPCELLPAKMGC18（100mm×3．0mm，3μm）色谱柱，流动相为甲醇-0．1％甲酸溶液（65：35），流速0．6mL·min^-1，柱温25℃，白术内酯Ⅰ及Ⅲ检测波长为220nm，白术内酯Ⅱ检测波长为280nm，进样量10μL。结果白术内酯Ⅰ的线性范围为1．128～56．42μg·mL^-1（r=0．9999）；白术内酯Ⅱ的线性范围为1．790～89．50μg·mL^-1（r=0．9999）；白术内酯Ⅲ的线性范围为1．268～63．42μg·mL^-1（r=0．9999）；平均加样回收率分别为98．5％（RSD=3．8％）、102．4％（RSD=2．8％）、98．6％（RSD=3．2％）。结论本方法简便、快速，测定结果准确可靠，可用于白术药材的质量控制。Abstract： Objective To establish a method for simultaneously determining atractylenolide Ⅰ , Ⅱ , and Ⅲ in Atrac- tylodes macrocephala Koidz.. Methods HPLC was used. The column was SHISEIDO CAPCELL PAK MG C18 （100 mm×3.0 mm, 3μm）, the mobile phase was methanol-0.1% formic acid solution = 65 ： 35 （v/v）, the flow rat was 0.6 mL·min ^-1, the temperature of column was 25 ℃ , and the detection wavelength was 220 nm for atractyleno- lide Ⅰ , Ⅲ , and 280 nm for atractylenolide Ⅱ , and the injection volume was 10 μL. Results Atractylenolide l had good linearity at 1.128 - 56.42 μg·mL^-1（r = 0.999 9）, and atractylenolide Ⅱ had good linearity at 1.790 - 89.50μg · mL^-1（r=0.999 9）. Atractylenolide Ⅲ was 1.268 - 63.42 μg·mL ^-1（r = 0.999 9）. The average recovery was 98.5% （RSD= 3.8%）, 102.4% （RSD = 2.8%） and 98.6% （RSD =3.2%）, respectively. Conclusion The method is simple, rapid and reliable for quality control ofAtractylodes macrocephala Koidz..

关 键 词：白术 含量测定 白术内酯Ⅰ 白术内酯Ⅱ 白术内酯Ⅲ 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]