固定化乳酸脱氢酶的催化性质研究  

Study on the Enzymatic Characteristics of Immobilized Lactate Dehydrogenase

作　　者：柳畅先[1] 宁静[1] 俸红梅 

机构地区：[1]中南民族大学 化学与材料科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2014年第1期15-18,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学自然科学基金资助项目(XTZ09005)

摘　　要：研究了固定化乳酸脱氢酶(LDH)的制备和催化性质,讨论了戊二醛体积分数、酶的偶联时间、pH值对酶固定化的影响.对游离酶和固定化酶催化性质的研究结果表明:酶促反应最适pH分别为9.2和10.2,最适温度分别为51℃和50℃,米氏常数分别为16.2 mmol/L和0.7 mmol/L.与游离酶相比,固定化酶的活力稳定性更佳,有更好的贮存稳定性和复用性.In this paper,the preparation and enzymatic characteristics of immobilized lactate dehydrogenase （ LDH） was studied and the impact of glutaraldehyde concentration , enzyme binding time and pH value on immobilization were discussed.The results of the study on the enzymatic characteristics of free and immobilized enzymes showed that the optimum pH value for the enzymatic reaction were 9.2 and 10.2,the optimum temperatures were 51℃ and 50℃,the Km value were 16.2 mmol/L and 0.7 mmol/L respectively.It was shown that the immobilized enzyme has better activity stability, storage stability and reusability .

关 键 词：乳酸脱氢酶 固定化 壳聚糖 酶催化性质 

分 类 号：TQ032[化学工程] O652[理学—分析化学]