HPLC-MS和HPLC-UV同时测定人血浆中阿斯匹林和水杨酸的浓度  

Determination of Aspirin and Salicylic acid in human Plasma by LC-MS and HPLC-UV

作　　者：张琪[1] 王广基[1] 孙建国[1] 吴民淑[1] PaulFawcett 

机构地区：[1]中国药科大学药物代谢中心,南京210009 [2]School of Pharmacy,University of Otago,Dunedin,NewZealand

出　　处：《中国药科大学学报》2001年第1期47-50,共4页Journal of China Pharmaceutical University

摘　　要：目的 :同时测定人血浆中阿斯匹林和水杨酸的浓度。方法 :用 HPLC-MS法测定人血浆中阿斯匹林 ,同时用 HPLC-UV法测定人血浆中水杨酸的浓度。结果 :阿斯匹林 ,水杨酸的线性范围分别为 2 0～ 1 0 0 0 ng/ml和 0 .1～ 2 0μg/ ml,最低检测浓度分别为 5.0 ng/ ml和 0 .1μg/ ml。方法的绝对回收率均大于 90 % ,高、中、低浓度的日内和日间变异系数均小于 1 0 %。结论 :该方法符合生物样品分析要求 ,可用于人体内药代动力学研究。AIM:To establish methods of HPLC MS and HPLC UV for the determination of aspirin (Asp) and its main metabolite salicylic acid (SA) in human plasma respectively. METHODS: acidified plasma was extracted by chloroform isopropanol (95∶5 v/v). Asp and SA were determined by HPLC MS (column: Kromasil ODS, 5 μm, 250 mm×4.6 mm ID) and HPLC UV (column: Hypersil ODS, 5 μm, 250 mm×4.6mm ID) respectively. RESULTS: The determination of Asp and SA in human plasma was as low as 5.0 ng/ml and 0.1 μg/ml respectively, with good recovery (better than 90%) and good precision (better than 10%). CONCLUSION: The assay was used to study the phamacokinetics and bioavailability of aspirin enteric coated control release tablets in healthy volunteers.

关 键 词：阿斯匹林 水扬酸 HPLC-MS 血药浓度 解热镇痛药 

分 类 号：R965[医药卫生—药理学]