大鼠脊髓半损伤后免疫荧光及超微结构的研究  

Study about immunofluorescence and ultrastructure after half damage in rat spinal cord

作　　者：孙淑红[1] 赵淑敏[2] 纪正春[1] 孔祥玉[2] 

机构地区：[1]承德医学院临床技能教学中心,承德067000 [2]承德医学院解剖学教研室,承德067000

出　　处：《中国免疫学杂志》2014年第2期195-197,201,共4页Chinese Journal of Immunology

摘　　要：目的:研究大鼠脊髓胸段半损伤后前角运动神经元区5-HT2AR免疫反应的变化,应用电镜观察胞体、髓鞘等结构的超微形态,为脊髓半损伤的病理、生理学变化提供形态学数据。方法:Wistar大鼠随机分成8 h、1、3、7、14、21、30 d七组,每一组又分为脊髓半损伤组和假手术组。脊髓半损伤组用改良Allens法制作大鼠胸段右半侧脊髓损伤模型,假手术组只去除椎板。经5-HT2A受体免疫荧光染色,用激光共聚焦显微镜观察摄片,电镜观察并摄片。结果:假手术组5-HT2A受体免疫反应阳性神经元的密度随时间的增加差异无统计学意义(P>0.05);脊髓半损伤组损伤侧与假手术组相比,损伤侧在1 d时5-HT2A受体阳性神经元的密度增加了2倍多,与假手术组相比差异有统计学意义(P<0.05),并随时间的增加而逐渐增高,与假手术组相比差异显著(P<0.01);脊髓半损伤后早期超微结构改变以细胞器的退行性改变为主,兼有膜结构的异常,时间延长则超微结构改变明显。后期损伤段以下脊髓逐渐失去正常神经组织结构,代之以神经胶质细胞增加。结论:脊髓胸段半损伤后,损伤下段前角运动神经元5-HT2A受体逐渐增高,并且出现了显著性差异。丰富了脊髓损伤后超微结构变化的理论。Objective：To study the 5-HT2A-IR density changes after injury of thoracic segments within the anterior horn motor neuron pool after hemi-contusion of the spinal cord.To observe the changes of neurons and myeline sheath on uhrastructural changes with electron microscope for providing that the pathophysiological changesmorphologically.Methods：56 Wistar rats were randomly divided into 7 groups：8 h,1 d,3 d,7 d,14 d,21 d and 30 d group （n =8）.The rats in different groups were also subdivided into a spinal semi-injury group and a sham-operation group （n =4）.In the spinal semi-injury group,the moderate SCI models were built based on modified Allens hit the right side damage model.In the sham-operation group,control only remove vertebral plate,but the spinal dura mater was kept intact,electron microscope was used to observe and photograph.Results：There was no significant difference statistically in the spinal cord anterior horn motor neurons in area 5-HT2A receptors （P ＞ 0.05） ; the density of 5-HT2AR-IR in the semi-contusion group were increased two times in the 1 day with the sham-operation group（P ＜0.05）,while its were increased significantly with the extension of the time（P ＜ 0.01）.In the early-stage after spinal semi-injury,the ultrastructural changes were priority to organelles degenerative changes and membrane structure abnormalities,the ultrastructural change significantly with the extension of the time.In the late-stage,the spinalcord has lost neural structures and was replaced by neuroglia.Conclusion：5-HT2AR-IR were increased in the contusion side obviously in motor neuron pool with remarkably difference,the basic theory about ultrastructure after spinal cord injury has been enriched in this study.

关 键 词：脊髓半损伤 免疫荧光 超微结构 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]