清络通痹颗粒干预类风湿关节炎破骨细胞分化相关因子分泌的研究  

Effects of Qingluo Tongbi granule on secretion of osteoclast differentiation related factors in rheumatoid arthritis

作　　者：周学平[1] 周玲玲[1] 陈晨[1] 柳璋璞[1] 刘天阳[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《中国免疫学杂志》2014年第2期202-204,208,共4页Chinese Journal of Immunology

基　　金：江苏省"六大人才高峰"项目(2007088);教育部博士点科研基金项目(20103237110004)资助

摘　　要：目的:通过观察清络通痹颗粒对类风湿关节炎破骨细胞分化相关因子分泌的影响,探讨其干预破骨细胞分化的分子机制。方法:建立佐剂性关节炎大鼠滑膜成纤维细胞和单核细胞共培养诱生破骨样细胞模型,制备清络通痹颗粒含药血清,设正常对照组、雷公藤多苷片组7.5 mg/kg、清络通痹颗粒大剂量组14.4 g生药/kg、中剂量组7.2 g生药/kg、小剂量组3.6 g生药/kg;将含药血清加入共培养体系,采用ELISA法检测滑膜成纤维细胞-单核细胞共培养诱生破骨细胞分化前、分化中、分化后培养上清中RANKL、IL-34、OPN的含量。结果:清络通痹颗粒各剂量组均能不同程度地降低破骨细胞分化前、分化中、分化后培养上清中RANKL、IL-34、OPN的水平,尤以大、中剂量组作用明显。结论:清络通痹颗粒可能通过降低RANKL上游分泌的IL-34、OPN等炎症细胞因子,抑制RANKL这一介导破骨细胞分化关键因子的过度表达,从而干预破骨细胞的分化,减轻类风湿关节炎关节软骨及软骨下骨破坏,延缓骨质破坏的进程。Objective：To study effects of Qingluo Tongbi Granule on inhibiting osteoclast differentiation in rheumatoid arthritis through decreasing osteoclast differentiation related factor.Methods：The synovial fibroblasts and monocytes of peripheral blood from adjuvant-induced arthritic rat were co-cultured to induce osteoclast-like cells.The rats were divided into five groups：control group,positive control group （tripterygium glycosides 7.5 mg/kg）,QLT high dose group （14.4 g/kg）,QLT middle dose group （7.2 g/kg）,QLT low dose group （3.6 g/kg）,and drug-containing sera were prepared and added to the co-cultured system.The levels of RANKL,IL-34 and OPN were detected by ELISA in the different period：before differentiation,during differentiation and after differentiation.Results：QLT-containing sera could reduce the levels of OPN,IL-34 and RANKL in the supernatants in different degree,and high and middle dose had obvious inhibitory effects.Conclusion：QLT could mediate the osteoclast RANKL expression by suppressing its upstream inflammatory cytokines such as IL-34 and OPN.Thus,it could delay the cartilage and bone destruction in RA.

关 键 词：清络通痹颗粒 类风湿关节炎 破骨细胞 细胞因子 

分 类 号：R285.5[医药卫生—中药学]