α-Thujone增强CD3AK细胞增殖及杀伤功能  

Augmentation of proliferation and cytotoxicity of CD3AK cells by α-Thujone

作　　者：周燏[1] 刘军权[1] 周忠海[1] 吕小婷[1] 张颂[1] 张娟[1] 陈复兴[1] 

机构地区：[1]中国人民解放军第九七医院肿瘤生物治疗中心,徐州221004

出　　处：《中国免疫学杂志》2014年第2期222-225,共4页Chinese Journal of Immunology

摘　　要：目的:研究α-Thujone对CD3AK细胞增殖及杀伤功能的影响。方法:从人外周血来源的PBMC按常规方法培养成CD3AK细胞,用不同浓度的α-Thujone处理CD3AK细胞,CCK-8法检测CD3AK细胞增殖及其对K562、SW480、HO8910三株肿瘤细胞的杀伤情况。FCM检测经α-Thujone处理后CD3+CD8+T细胞上CD107a的表达。结果:α-Thujone能显著促进CD3AK细胞的增殖,表现出一定的浓度依赖性。α-Thujone还能促进细胞CD107a的表达和对多种肿瘤细胞的杀伤活性。结论:α-Thujone能促进CD3AK细胞增殖并增强其细胞毒性。Objective：To investigate the effect of α-Thujone on CD3AK cells proliferation and cytotoxicity.Methods：CD3AK cells were cultured from PBMC and treated with different concentrations of α-Thujone.The proliferation and cytotoxicity assay were detected by CCK-8 kit.The expression of CD107a on CD3 ＋ CD8 ＋ T cell was determined by FCM.Results：α-Thujone could significantly improve CD3AK cells proliferation and cytotoxicity to K562,SW480,HO8910 cells,and enhance the expression of CD107a.Conclusion：α-Thujone could significantly improve CD3AK cells proliferation and cytotoxicity.

关 键 词：α-Thujone CD3AK CD107a 细胞毒性 

分 类 号：R979.5[医药卫生—药品]