血必净对脂多糖作用下大鼠腹膜间皮细胞HMGB-1、RAGE及TGF-β1表达的影响  

Expression of HMGB-1,RAGE and TGF-β1 in rat peritoneal mesothelial cells induced by Lipopolysaccharide,Xuebijing

作　　者：陈硕[1] 高利丽[1] 王二敏[1] 周红丽[1] 

机构地区：[1]辽宁医学院附属第一医院肾内科,锦州121000

出　　处：《中国免疫学杂志》2014年第2期266-270,共5页Chinese Journal of Immunology

摘　　要：目的:观察血必净对脂多糖(LPS)作用下大鼠腹膜间皮细胞(PMC)高迁移率族蛋白-1(HMGB-1)、糖基化终产物受体(RAGE)及转化生长因子-β1(TGF-β1)表达的影响。方法:胰蛋白酶消化法用于PMC的原代培养和传代,经鉴定第三代细胞用于实验并随机分组:正常对照组;不同浓度LPS组(1、10、100 mg/L);不同时间组:10 mg/L LPS作用于PMC 24、36、48 h;血必净组:10 mg/L LPS预孵育2 h加入血必净10 g/L再作用34 h。用RT-PCR法检测RAGE-mRNA的表达,Western blot检测RAGE蛋白的表达,ELISA法检测细胞培养上清液中HMGB-1和TGF-β1的蛋白表达。结果:LPS能显著上调PMC RAGE-mRNA和蛋白的表达(P<0.05),且随LPS刺激时间的延长及LPS浓度的增加,RAGE表达增加;LPS刺激PMC HMGB-1、TGF-β1蛋白表达显著高于正常对照组,差异有统计学意义(P<0.05);血必净可抑制LPS所致的HMGB-1和RAGE的表达,并下调TGF-β1的表达。结论:HMGB-1与其受体RAGE可能通过上调TGF-β1参与腹膜纤维化的发生,而血必净下调HMGB-1、RAGE及TGF-β1的表达,为临床防治腹膜纤维化提供新思路。Objective：To observe the effect of Xuebijing on the expression of high mobility group box-1 protein and receptor for advanced glycation end products; high mobility group box-1 and transforming growth factor-β1 in rat peritoneal mesothelial cells （PMCs） induced by Lipopolysaccharide （LPS）.Methods：PMCs were isolated from rat colic omentum and the 3rd generation cells were used in the experiment：cells were incubated with LPS in different concentrations （1,10,100 mg/L） ; with LPS （10 mg/L） for 24,36,48 h; with Xuebijing in 10 g/L after incubation with LPS （10 mg/L） for 36 h.The control group were incubated with medium.RAGE mRNA was detected by RT-PCR.RAGE protein was detected by Western blot,HMGB-1 and TGF-β1 were detected by ELISA in supematants.Results：Compared with the control group,the expression of RAGE-mRNA and protein were significantly increased in groups stimulated by LPS in a time-dependent and dose-dependent manner（all P ＜ 0.05）.The expression of HMGB-1 and TGF-β1 were increased in the groups stimulated by LPS in a time-dependent manner（P ＜ 0.05）.HMGB-1 and RAGE may be involved in upregulating the expression of TGF-β1.Compared to LPS control group,Xuebijing significantly inhibited the expression of HMGB-1,RAGE and TGF-β1 （all P ＜ 0.05）.Conclusion：HMGB-1 and RAGE may be involved in the pathogenesis of peritoneal fibrosis by upregulating the expression of TGF-β1.However Xuebijing may have some peritoneal protective effect,partly through inhibition of excessive expression of HMGB-1 and RAGE.

关 键 词：脂多糖 细胞 糖基化终产物受体 高迁移率族蛋白-1 转化生长因子-β1 

分 类 号：R459.5[医药卫生—治疗学]