不同采收期广东紫珠中两种抗炎活性成分的动态变化  

Dynamic Variation of Two Anti-inflammatory Components in Gallicarpa kwangtungensis Chun. from Different Collection Time

作　　者：陈颖[1] 许敏[1] 凌嘉伟 王晓丹[1] 程雨兰 刘丽芳[1] 

机构地区：[1]中国药科大学天然药物活性组分与药效国家重点实验室,江苏南京210009

出　　处：《中医药学报》2014年第1期29-31,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家级大学生创新创业训练计划项目(No.G12126)

摘　　要：目的：采用HPLC法测定不同采收期广东紫珠（Gallicarpa kwangtungensis Chun.）中抗炎活性成分连翘酯苷B和金石蚕苷的含量,研究这两种活性成分动态变化,为药材最佳采收期的确定提供依据。方法：色谱条件：色谱柱为DiamonsilC18色谱柱（150mm×4.6mm,5μm）;流动相为乙腈-0.5%磷酸溶液（17：83）等度洗脱;流速为1.0mL/min;检测波长为332nm;柱温为30℃。结果：连翘酯苷B及金石蚕苷的线性范围分别是0.051-4.696μg（r=0.999 7）、0.028-0.767μg（r=0.999 7）,平均加样回收率为97.18%（RSD=0.93%）、99.24%（RSD=1.54%）。结论：研究得出该产区广东紫珠的最佳采收期为7月和11月。Objective： The dynamic variations of two anti- inflammatory components,forsythiaside B and poliumoside,in Gallicarpa kwangtungensis Chun. from different collection time were investigated in this work by HPLC. Method： The separation was performed in a Diamonsil C 18 column（ 150mm × 4. 6mm,5μm） with a mobile phase of acetonitrile-0. 5% phosphoric acid（ 17： 83）. The flow rate was 1. 0mL / min,and the detecting wavelength was 332nm. The temperature of the column was 30℃. Results： The linear range of forsythiaside B and poliumoside were 0. 051 ~ 4. 696μg（ r = 0. 999 7） and 0. 028 ~ 0. 767μg（ r = 0. 999 7）,respectively. The average recoveries of them were 97. 18%（ RSD = 0. 93%） and 99. 24%（ RSD = 1. 54%）. Conclusion： The best collection time is from July to November.

关 键 词：HPLC 广东紫珠 连翘酯苷B 金石蚕苷 

分 类 号：R284.1[医药卫生—中药学]