α-玉米赤霉醇对大鼠成骨细胞IL-6表达及分泌的影响  

Effects of α-zeranol on the IL-6 expression and secretion in rat osteoblasts

作　　者：段斐[1] 郝华超[1] 薛娟[1] 李少春[1] 刘未华[1] 谷雨[1] 孙晓芳[1] 

机构地区：[1]河北大学基础医学院,河北保定071000

出　　处：《医学研究与教育》2014年第1期1-7,共7页Medical Research and Education

基　　金：河北大学医学学科建设项目(2012A2002);河北省计生委基金项目(2010A03)

摘　　要：目的：探讨α-玉米赤霉醇对体外培养的大鼠成骨细胞IL-6表达及分泌的影响。方法体外培养原代SD新生大鼠颅骨成骨细胞，形态学观察、碱性磷酸酶（ALP）染色、I型胶原免疫荧光染色进行成骨细胞鉴定，分别以雌激素及不同浓度的α-玉米赤霉醇作用于成骨细胞，采用RT-PCR法检测IL-6 mRNA的表达，ELISA法检测成骨细胞培养上清中IL-6的含量。结果10^-10-10^-5mol/Lα-玉米赤霉醇能够抑制成骨细胞IL-6 mRNA的表达及其蛋白分泌，其中以10-6 mol/L、10-7mol/L的α-玉米赤霉醇作用显著。结论α-玉米赤霉醇能够抑制成骨细胞细胞因子IL-6 mRNA及蛋白的表达，这可能是α-玉米赤霉醇防治绝经后骨质疏松症的机制之一。Objective To investigate the effects ofα-zeranol on the IL-6 expression and secretion in rat osteoblasts. Methods Osteoblasts were isolated from the skull of SD neonatal rats and identified by morphological observation, ALP staining, and collagen I immunolfuorescence staining. 17β-estradiol andα-zeranol with different concentration were administrated. The expression of IL-6 mRNA in the osteoblasts was detected by RT-PCR and the level of IL-6 in culture supernatant of osteoblasts was measured by ELISA. Results α-zeranol with 10^-10-10^-5 mol/L concentrations could inhibit the expression and secretion of IL-6 in osteoblasts, among which the concentrations of 10-6 and 10-7 mol/L had obvious effects. Conclusionα-zeranol can inhibit the expressions of IL-6 mRNA and protein in osteoblasts, it may play an important role in the prevention of postmenopausal osteoporosis.

关 键 词：Α-玉米赤霉醇 成骨细胞 白细胞介素6 绝经后骨质疏松症 IL-6 

分 类 号：R977[医药卫生—药品]