柴胡桂枝汤对肝纤维化大鼠FN、a-SMA表达的影响  

Effect of Chaihu Guizhi Decoction on Expression of FN and a-SMA of Rats with Liver Fibrosis

作　　者：李林蔚[1] 周景华[2] 刘华生[1] 

机构地区：[1]黑龙江中医药大学中医基础诊断教研室,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《黑龙江医药》2014年第2期265-267,共3页Heilongjiang Medicine journal

基　　金：黑龙江省研究生创新科研资助项目（No.D2005-37）

摘　　要：目的:观察柴胡桂枝汤对肝纤维化大鼠纤维连接蛋白(FN)和a-平滑肌动蛋白(a-SMA)表达的影响。方法:采用CCl4复合法制备肝纤维化大鼠模型;用免疫组化方法观察实验动物模型肝脏中FN、a-SMA表达的情况。结果:柴胡桂枝汤可抑制肝纤维化大鼠肝脏中FN、a-SMA的表达,中药中等浓度组FN和a-SMA的表达水平明显低于模型组、中药高剂量组、中药低剂量组和西药对照组,具有极显著性差异(P<0.01)。结论:柴胡桂枝汤能够有效的控制纤维化肝脏中FN、a-SMA的高表达,抑制HSC活化,具有抗肝纤维化的作用。Objective： To investigate the effect of Chaihu Guizhi decoction on the expression of fibronectin（FN） and alpha smooth musele actin（a-SMA） in rats with liver fibrosis. Methods： Rat models of liver fibrosis were made by CC14 compound method.The effect of Chaihu Gnizhi decoction on the expression of FN and a-SMA of rats with liver fibrosis were observod by immunohistochemical method. Results： Chaihu Guizhi decoction could control the expression of FN and a-SMA in rats with liver fibrosis. Compared with the model group and high concentration of Chinese medicine group and low concentration of Chinese medicine group and Western Medi- cine group, the expression level of Fn and a-SMA in middle concentration of Chinese medicine group possessed a significant difference （ P 〈 0.01）. Conclusion： Chaihu Guizhi decoction can control the high expression levels of FN and a-SMA in rats with liver fibrosis, Inhibition of HSC was activated,and can plays an important role in the recovery of liver fibrosis.

关 键 词：肝纤维化 柴胡桂枝汤 FN A-SMA 

分 类 号：R285.5[医药卫生—中药学]