兔内皮祖细胞在聚乳酸 聚乙醇酸上生长分化促进糖尿病兔创面血管化的研究  

Study of growth and differentiation of rabbit endothelial progenitor cells in poly(lactic-co-glycolic acid) on promoting diabetic wound vascularization

作　　者：杨文健[1] 焦蓉[1] 

机构地区：[1]湖北医药学院附属襄阳医院内分泌科,441000

出　　处：《安徽医学》2014年第3期272-274,共3页Anhui Medical Journal

摘　　要：目的体外培养兔内皮祖细胞(EPCs)并种植于聚乳酸-聚乙醇酸(PLGA)上培养形成移植物,植入兔体内,观察其血管化的程度,探讨其促进糖尿病创面血管化的可行性。方法体外分离、培养、鉴定兔EPCs,并将其种植于PLGA上,形成细胞-支架复合移植物;造兔糖尿病模型,植入复合移植物的为实验组,仅植入支架材料的为对照组,分别于4周、8周后处死动物,复合移植物行组织学及免疫组化检测,观察血管化程度。结果术后8周,实验组移植物生长良好,可见较多的功能性新生血管穿插长入其中,对照组未见明显的血管生成(P<0.05)。结论 EPCs参与组织修复及促进新生血管生成,为糖尿病创面的愈合提供了血管化的基础。Objective Rabbit EPCs were cultured in vitro and planted on PLGA to form the grafts,which were then implanted in rabbits to observe the degree of vascularization,which may promote the cure of diabetic vascular wound.Methods In vitro,we isolated,cul-tured,identified the rabbit EPCs,and planted these cells on the PLGA to form cell-scaffold grafts;then diabetic rabbit model was made,and composite graft was implanted in the experimental group,only the scaffold ones for the experimental group.Respectively,after 4 and 8 weeks, the animals were sacrificed;in order to observe the vascularization of complex,the composite graft was detected by histological and immuno-histochemical methods.Results After 8 weeks,the experimental group graft material grew well,which were seen more functional neovascular-ization interspersed among it and in the control group,the scar contracture occurred with no significant angiogenesis.Conclusion EPCs are involved in tissue repair and promote angiogenesis,which provides a vascularized foundation for the healing of diabetes wound.

关 键 词：内皮祖细胞 聚乳酸-聚乙醇酸 糖尿病 血管化 

分 类 号：R587.2[医药卫生—内分泌]