金仁陈子方对IBS模型大鼠的治疗作用及机理研究  

Therapeutic Effect of Jinren Chenzi Formula on Irritable Bowel Syndrome Model Rat and Its Possible Mechanism

作　　者：张蓓[1] 袁凤娟 洪煜鹏 尹西拳[1] 吴依娜[1] 李震华[1] 刘小虹[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广东东泰乳业有限公司,广东揭阳515500

出　　处：《中药新药与临床药理》2014年第2期149-152,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省教育部产学研结合项目(2011B090400167)

摘　　要：目的观察金仁陈子方对母子分离复合应激诱导的肠易激综合征(IBS)大鼠的治疗作用,并探讨其作用机理。方法采用新生大鼠母子分离与避水应激复合法复制内脏高敏感性IBS大鼠模型,观察金仁陈子方对IBS大鼠内脏敏感性、避水应激排便粒数、血清一氧化氮(NO)含量、血清及结肠组织5-羟色胺(5-HT)含量的影响。结果与模型组比较,金仁陈子方高、低剂量组大鼠内脏敏感性显著降低(P<0.01),避水应激排便粒数显著减少(P<0.01),血清NO含量显著升高(P<0.01),血清及结肠组织5-HT含量均显著降低(P<0.05)。结论金仁陈子方可通过调节大鼠体内NO、5-HT水平,显著改善IBS模型大鼠肠道功能。Objective To observe the therapeutic effect of Jinren Chenzi formula on the model rats of irritable bowel syndrome induced by maternal separation combined with multiple stresses, and to investigate its possible mechanism. Methods IBS rat model with visceral hypersensitivity was established by neonatal maternal separation and water-avoidance stress , in order to study the effect of Jinren Chenzi formula on rat visceral hypersensitivity, water stress-induced defecation grains , serum contents of NO and 5-HT, and colonic 5-HT content. Results Compared to the model group , high- and low-dose Jinren Chenzi formula could significantly decrease the visceral hypersensitivity of IBS rats（P 〈 0.01 ）, obviously reduce the water stress-induced defecation grains（P 〈 0.01 ）, significantly increase serum NO content（P 〈 0.01） and decrease the 5-HT content in serum and colon obviously （P 〈 0.05）. Conclusion Jinren Chenzi formula can effectively improve gastrointestinal disorders of IBS rats, and its therapeutic mechanism may be related to regulating NO and 5-HT level.

关 键 词：金仁陈子方 肠易激综合征 母子分离 -氧化氮 5-羟色胺 

分 类 号：R285.5[医药卫生—中药学]