加味参附颗粒对慢性心力衰竭大鼠血流动力学及心肌能量代谢的影响  

Influence of Modified Shenfu Granules on Hemodynamics and Myocardial Energy Metabolism in Chronic Heart Failure Rats

作　　者：史振羽[1] 靳利利[1] 袁丁[1] 姚楠[1] 王清海[1] 荣尚一[1] 

机构地区：[1]广东省第二中医院,广东广州510095

出　　处：《中药新药与临床药理》2014年第2期172-176,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东科技计划课题(2010B030700021)

摘　　要：目的观察加味参附颗粒对慢性心力衰竭大鼠的血流动力学、心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性的影响。方法雄性SD大鼠50只,随机分为5组:正常对照组,模型组,曲美他嗪组及加味参附颗粒高、低剂量组,每组各10只。采用腹主动脉缩窄法建立心衰模型,预处理8周后,测定心衰大鼠心率、左室内最大上升和下降速率(±dp/dtmax)、血压、心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性。结果加味参附颗粒高、低剂量组心率、血压均低于模型组(P<0.05,P<0.01),在改善心率方面优于曲美他嗪组(P<0.05)。加味参附颗粒高、低剂量组±dp/dtmax、心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性均高于模型组(P<0.05);与曲美他嗪组比较,差异无统计学意义(P>0.05)。结论加味参附颗粒可能通过改善心肌能量代谢,进而改善心衰大鼠血流动力学,提高心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性。Objective To observe the influence of modified Shenfu granules （MSG） on chronic heart failure rat hemodynamics, myocardial mitochondrial protein concentration and the activities of Na＋-K＋-ATPase, Ca2＋-ATPase. Methods Fifty male SD rats were evenly and randomly divided into normal control group, model control group, western medicine group（treated with trimetazidine）, and high-dose and low-dose MSG groups. Rat heart failure model was established by abdominal aortic coarctation method. After treatment for 8 weeks, heart rate, the maximum left ventricular pressure increase and decrease rate （＋dp/dtmax）, blood pressure, cardiac mitochondrial protein concentration, and the activities of Na＋-K＋-ATPase and Ca2＋-ATPase of heart failure rats were measured. Results Heart rate and blood pressure in high, and low-dose MSG groups were lower than those in the model group（P 〈 0.05, P 〈 0.01）, and MSG had better effect on improving heart rate than trimetazidine （P 〈 0.05）. High-, and low-dose MSG groups had better effect on increasing _＋ dp/dtn＋,）, cardiac mitochondrial protein concentration, and the activities of Na＋-K＋-ATPase and Ca2＋-ATPase than the model group （P 〈 0.05）, and the differences had no statistical significance when compared with trimetazidine group（P 〉 0.05）. Conclusion MSG ean improve the hemodynamics of heart failure rats, and increase myocardial mitochondrial protein concentration and the activities of Na＋-K＋-ATPase through the improvement of myocardial energy metabolism.

关 键 词：加味参附颗粒 心力衰竭 血流动力学 能量代谢 大鼠 

分 类 号：R285.5[医药卫生—中药学]