HPLC法测定灵芝孢子油中1,2-二油酸-3-棕榈酸甘油酯含量  

Determination of 1,2-Dioleoyl-3-palmitoyl glycerol in Ganoderma lucidum Spore Oil by HPLC

作　　者：万明珠[1] 王腾华[1] 张丽媛[1] 任灵芝[1] 谭敏[1] 高幼衡[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2014年第2期218-221,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立高效液相色谱法（HPLC）测定灵芝孢子油中l，2-二油酸-3-棕榈酸甘油酯含量的方法。方法采用HPLC法，色谱柱：DiamonsilC1。（250mln×4．6mm，5μm）柱，流动相：乙腈-异丙醇（体积比为5l：49），流速：1．0mL·min-1，ELSD漂移管温度：40℃，载气流速：2．4L·min-1。结果l，2-二油酸-3-棕榈酸甘油酯在2．7～27．0μg范围内线性关系良好（r=0．9996），平均回收率为99.3％，RSD值为23％。结论该方法简便、准确、分离度好，可用于灵芝孢子油中1，2-二油酸-3-棕榈酸甘油酯的含量测定。Objective To establish a method for the content determination of 1, 2-dioleoyl-3-palmitoyl glycerol inGanoderma lucidum spore oil by HPLC. Methods HPLC was performed on a Diamonsil Cls column （250 mm X4.6 mm, 5 μm） with acetonitrile-isopropanol （51 ： 49） as the mobile phase and the flow rate was 1.0 mL min-1. The temperature of drift tube was 40 ℃, and the flow rate of gas was 2.4 L min-1. Results The calibration curves of 1, 2-dioleoyl-3-palmitoyl glycerol were in good linearity in the range of 2.7-27.0 μg（r=0.9996）. The average recovery was 99.3 %, RSD being 2.3 %. Conclusion The method is simple, accurate and reliable with a good resolution, and can be used for the quantitative determination of 1, 2-dioleoyl-3-palmitoyl glycerol in Ganoderma lucidum spore oil.

关 键 词：灵芝孢子油 l 2-二油酸-3-棕榈酸甘油酯 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]