特应性皮炎患儿外周血巨噬细胞游走抑制因子及IL-17和IL-23的表达及其相关性研究  

Expressions of Macrophage Migration Inhibitory Factor,IL-17,IL-23 in Children with Atopic Dermatitis and Their Correlation

作　　者：马蕾[1] 高梅兰[2] 薛海波[3] 管秀好[4] 舒春梅[1] 张桂芹[1] 运蓓蕾[5] 

机构地区：[1]滨州医学院附属医院皮肤科,山东省滨州市256603 [2]滨州医学院附属医院检验科,山东省滨州市256603 [3]滨州医学院临床学院内科学教研室 [4]中国医科大学附属第一医院皮肤科 [5]沈阳市第七人民医院皮肤科

出　　处：《中国全科医学》2014年第8期891-894,共4页Chinese General Practice

基　　金：山东省科技发展计划(2011YD18069);山东省自然科学基金资助项目(ZR2010HQ013);山东省高等学校科技计划(J10LF90);山东省医药卫生科技发展计划(2011QZ003);滨州市科技发展计划(2011ZC0914)

摘　　要：目的检测特应性皮炎(AD)患儿外周血单个核细胞(PBMCs)中巨噬细胞游走抑制因子(MIF)、IL-17和IL-23 mRNA表达水平及血清水平,并探讨其相关性。方法选择2010年4—11月在我院门诊就诊的36例AD患儿为病例组,同期在我院体检正常儿33例为对照组,采用实时荧光定量反转录聚合酶链反应(RT-PCR)检测两组受试者PBMCs中MIF、IL-17和IL-23 mRNA表达水平,酶联免疫吸附试验检测其血清MIF、IL-17和IL-23水平。结果病例组PBMCs中MIF、IL-17和IL-23 mRNA表达水平分别为(5.93±2.35)、(6.76±1.87)和(3.21±0.73),对照组分别为(1.83±0.69)、(1.77±0.51)和(0.92±0.22),病例组PBMCs中MIF、IL-17和IL-23mRNA表达水平均高于对照组(P<0.01)。病例组血清MIF、IL-17和IL-23水平分别为(34.92±8.73)μg/L、(33.29±5.70)ng/L和(54.62±9.69)ng/L,对照组分别为(9.80±2.21)μg/L、(11.71±2.08)ng/L和(18.97±4.39)ng/L,病例组血清MIF、IL-17和IL-23水平均高于对照组(P<0.01)。线性相关分析结果显示,AD患儿PBMCs中MIF mRNA表达水平(r=0.418,P=0.011)及血清MIF水平(r=0.419,P=0.011)与疾病严重程度(SCORAD)评分均呈正相关;PBMCs中MIF mRNA表达水平与IL-17 mRNA、IL-23 mRNA表达水平均呈正相关(rIL-17 mRNA=0.395,P=0.017;rIL-23 mRNA=0.422,P=0.010);血清MIF水平与IL-17、IL-23水平均呈正相关(rIL-17=0.407,P=0.014;rIL-23=0.375,P=0.024)。结论 MIF在AD患儿中高表达,且与IL-17、IL-23密切相关,炎性因子间的相互作用可能共同参与了AD的发生、发展。Objective To detect the expressions of macrophage migration inhibitory factor（MIF）,IL-17,IL-23 in peripheral blood mononuclear cells（PBMCs） and serum levels,and their correlation in children with atopic dermatitis（AD）.Methods Thirty-six AD children who visited this hospital from April to November 2010 were enrolled as study group,33 healthy children as control group.The expressions of MIF,IL-17,IL-23 in PBMCs were detected by real-time quantitative RT-PCR,levels of serum MIF,IL-17,IL-23 by enzyme-linked immunosorbent assay.Results The expressions of MIF,IL-17,IL-23 in PBMCs were higher in study group[（5.93±2.35）,（6.76±1.87）,（3.21±0.73）,respectively]than in control group[（1.83±0.69）,（1.77±0.51）,（0.92±0.22）,respectively];the serum levels of MIF,IL-17,IL-23 higher in study group[（34.92±8.73）μg/L,（33.29±5.70）ng/L,（54.62±9.69）ng/L,respectively]than in control group[（9.80±2.21）μg/L,（11.71±2.08）ng/L,（18.97±4.39）ng/L,respectively].By linear correlation analysis,MIF mRNA expression in PBMCs,serum MIF level were positively correlated with disease severity（SCORAD） score（r=0.418,P=0.011;r=0.419,P=0.011）;MIF mRNA expression in PBMCs positively correlated with expressions of IL-17,IL-23（rIL-17 mRNA=0.395,P=0.017;rIL-23 mRNA=0.422,P=0.010）;serum MIF level positively correlated with IL-17,IL-23（rIL-17=0.407,P=0.014;rIL-23=0.375,P=0.024）.Conclusion In AD children,MIF expression is high and closely correlated with IL-17,IL-23.The interaction between inflammatory cytokines may be involved in the occurrence and development of AD.

关 键 词：皮炎 特应性 巨噬细胞游走抑制因子 白细胞介素17 白细胞介素23 

分 类 号：R75[医药卫生—皮肤病学与性病学]