高效液相色谱法测定醋氯芬酸缓释片的含量  

Determination of aceclofenac in extended-released tablets by HPLC

作　　者：王槐武 黄励[2] 梅之南[3] 

机构地区：[1]湖北黄石市国药有限公司,黄石435000 [2]大冶有色金属公司职工医院 [3]湖北医科大学附一院药学部

出　　处：《中国现代应用药学》2001年第1期53-55,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 :建立了高效液相色谱法测定醋氯芬酸缓释片的含量。方法 :采用ZorbaxSB C18色谱柱 ,以乙腈 四氢呋喃 冰醋酸(2 5∶2 5∶5 0 ,用 1.0mol/L的NaOH调pH 3.5 )为流动相 ,流速为 1.0ml/min ,以对羟基联苯为内标物 ,检测波长为 2 75nm。结果 :醋氯芬酸在 10 .2～ 5 0 .1μg/ml范围内呈良好线性 (r=0 .9993) ,平均回收率为 10 0 .3% ,RSD为 0 .45 %。 结论 :本法可用于该片剂的测定 ,操作简便 ,结果准确。OBJECTIVE:To establish a HPLC method for the determine of aceclofenac extended tablets.METHODS:A Zorbax SB C 18 column was used.The mobile phase was acetonitrile tetrahydrofuran glacial acetic acid(25∶25∶50,adjusted to pH 3.5 with 1.0 mol/L NaOH) and the flow rate was 1.0ml/min,intertal standard was para hydroxybiphenyl.The UV detection wavelength was 275nm.RESULTS:The calibration curve was linear in the range from 10.2μg/ml to 50.1μg/ml(r=0.9993) and the average recovey was 100.3%(RSD=0.45%).CONCLUSION:The method is selective,simple and accurate.

关 键 词：高效液相色谱法 醋氯芬酸缓释片 含量测定 非甾体抗炎药 

分 类 号：R971.1[医药卫生—药品] R927.2[医药卫生—药学]