双波长比值光谱法测定银黄口服液中绿原酸和黄芩苷含量  

Determination of chlorogenic acid and baicalin in Yinhuang oral solution by dual-wavelength ration spectrometry

作　　者：孙增先[1] 李永溟[1] 李瑞真[1] 王奎兴[1] 张骞峰[1] 

机构地区：[1]江苏连云港市第一人民医院,连云港222002

出　　处：《中国现代应用药学》2001年第1期55-57,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 :应用双波长比值光谱法对银黄口服液中绿原酸和黄芩苷含量测定进行研究。方法 :依据绿原酸和黄芩苷的比值光谱特征 ,选择 2 6 6和 34 2nm作为测定波长。结果 :绿原酸线性范围 2～ 2 0 μg/ml,回收率 98.9,RSD为 1.5 8% ;黄芩苷线性范围2～ 16 μg/ml,回收率 10 0 .4% ,RSD为 1.32 %。 结论 :本法为一种灵敏、准确和简便的分析方法 ,可在其他中药制剂中推广应用。OBJECTIVE:To determine of chlorogenic acid and baicalin in Yinhuang oral solution by dual wavelength ration spectrometry.METHODS:With the feature of ratio spectrometry for chlorogenic acid and baicalin,266 and 342nm were chosen as their determination wavelengthes.RESULTS:Linear range of chlorogenic acid and baicalin were 2～20μg/ml and 2～10μg/ml respectively.The recovery of chlorogenic acid and baicalin rate were 98.9%(RSD=1.58%) and 100.4%(RSD=1.32%) respectively.CONCLUSION:As a precise,accute and sample method,it could be spread to the assay of other traditional Chinese medicine products.

关 键 词：双波长经值光谱法 银黄口服液 绿原酸 黄芩苷 含量测定 

分 类 号：R286.0[医药卫生—中药学]