益母草注射液提取物对流产大鼠离体子宫的影响  

Effects of extract from Leonurus japonicus Injection on uterus of rats after abortion

作　　者：李丹[1] 谢晓芳[1] 彭成[1] 熊亮[1] 赵小梅[1] 刘娟[1] 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《药物评价研究》2014年第1期21-24,共4页Drug Evaluation Research

基　　金：国家"十一五"重点科技支撑计划项目(2009BAI84B00)

摘　　要：目的研究益母草注射液浸膏及其提取部位对大鼠流产子宫的作用。方法采用化学提取分离方法由益母草注射液中提取分离得到益母草生物碱部位(LH-1-B)、非生物碱部位(LH-1-A)。早孕大鼠ig米非司酮(8.3 mg/kg)和米索前列醇(100μg/kg)造成不完全流产,24 h后颈椎脱臼处死动物,迅速剖取子宫,用BL-420E生物机能实验系统记录大鼠离体子宫平滑肌的收缩活动;采用累积给药方式,分别给予益母草注射液浸膏(37.5、37.5、150.0μL)或LH-1-A、LH-1-B(75.0、75.0、150.0μL),每次给药后记录10 min收缩曲线,比较给药前后收缩张力、频率和活力的变化。结果益母草注射液浸膏可使流产大鼠离体子宫的活力和收缩张力增强(P<0.01);LH-1-A使流产大鼠离体子宫收缩活力、张力及频率降低(P<0.05、0.01);LH-1-B高剂量具有增加流产子宫收缩张力最小值作用(P<0.05)。结论益母草注射液浸膏可显著增强流产后大鼠子宫收缩活动,但其内含有促进流产子宫收缩和抑制流产子宫收缩的活性部位,其中水溶性生物碱部位是其缩宫的主要部位,作用机制有待进一步研究。Objective To study the effects of the extract from Leonurusjaponicus Injection （LJI） on uterus of rats after abortion. Methods The alkaloids fraction （LH-l-B） and non-alkaloid fraction （LH-l-A） were separated from LJI by chemical extraction. Pre pregnancy rats were ig administered with iVlifepristone （8.3 mg/kg） and Misoprostol （100 μg/kg） to induce incomplete abortion model. After 24 h, the rats were executed with cervical dislocation and the uterus was taken. The contraction activity of uterine smooth muscle in rats was recorded by BL--420E Biological Function Experimental System. The doses of extracts were 37.5, 75, and 150 IxL and the doses of LH- 1-A and LH-1-B were 75, 150, and 300 μL by using cumulative mode of administration. The constriction curve for 10 min was recorded including the amplitude, frequency, and mobility in uterine smooth muscle movement track. Results The extract from LJI could enhance the activity of abortion uterus （P 〈 0.01）; The LH- 1-A extract could decrease the activity of abortion uterus （P 〈 0.05 or 0.01）; The high-dose LH-1-B could increase the minimum of uterus contraction （P 〈 0.05）. Conclusion The extract from LJI could significantly enhance the contraction of uterus in rats after abortion, and contains the components with the effects of promoting and inhibiting contraction of uterus. The alkaloid fraction is the main part with the promoting effect. Further investigations are needed to explore the action mechanism.

关 键 词：益母草注射液 流产子宫 生物碱 非生物碱 

分 类 号：R965.1[医药卫生—药理学]