种植人体活性细胞的生物心脏瓣膜  

Bioprosthetic Heart Valves Implanted with Viable Human Cells

作　　者：张祖仁[1] 王一山[1] 

机构地区：[1]上海第二医科大学附属仁济医院心血管外科研究室,上海200001

出　　处：《中国胸心血管外科临床杂志》2001年第1期50-53,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

摘　　要：目前 ,种植人体活性细胞的生物心脏瓣膜主要有组织工程心脏瓣膜和种植人体活性细胞的猪主动脉瓣两种。组织工程心脏瓣膜是在人体可吸收的聚二醇酸纤维支架上种植人体同种活性细胞 ,先种植成纤维细胞 ,再种植单层内皮细胞包裹瓣叶。种植人体活性细胞的猪主动脉瓣是在清除原有细胞的组织内重建人体同种活性细胞。清除新鲜猪主动脉瓣组织内原有细胞的方法是将瓣膜先经高、低渗溶液处理 ,然后用酶溶液处理。细胞经培养分离后 ,将成纤维细胞植入经处理的瓣膜组织 ,再植入内皮细胞。种植人体活性细胞的生物心脏瓣膜不会促使受者产生有害的免疫反应 ,并具有再生能力。Current bioprosthetic heart valves implanted with viable human cells can be categorized into two basic designs, tissue engineering heart valve and porcine aortic valve implanted with viable human cells. Tissue engineering heart valve is constructed by seeding a synthetic polyglycolic acid fiber based biodegradable scaffold with viable human autologous cells. The fibroblasts are first seeded on valve scaffold. When the fibroblasts reached confluence, endothelial cells are seeded onto the surface of leaflets and grown into a single monolayer. Porcine aortic valve implanted with viable human cells is generated by removing native cells from tissue and then repopulating the tissue with viable human autologous cells. The method of removing native cells from fresh porcine aortic valve comprises subjecting the valve tissue to a hypotonic/hypertonic treatment, then treating the valve tissue with an enzyme based solution. Isolated cell lines are cultured and thereafter such a valve matrix is implanted with fibroblasts, followed by endothelial cells. Bioprosthetic heart valves implanted with viable human cells do not produce and adverse immune response by the recipient upon implant, and possesses the regenerative capabilities.

关 键 词：生物瓣 人体活性细胞 组织工程 猪主动脉瓣 清除细胞 种植细胞 

分 类 号：R318.1[医药卫生—生物医学工程]