黄芩苷通过抑制核因子-κB表达减轻胆管不全梗阻大鼠肠黏膜损伤  

Protective effects of Baicalin on intestinal mucosa injury by inhibiting nuclear factor-KB expression in rats with partial common bile duct ligation

作　　者：刘希杰[1] 冯文玉[1] 耿磊[1] 刘腾[1] 郑步峰[1] 傅廷亮[1] 

机构地区：[1]滨州医学院附属医院儿外科,山东滨州256603

出　　处：《中华实用儿科临床杂志》2014年第7期514-517,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：滨州医学院科技计划项目（BY2013KJ20、BY2012KJZD02）

摘　　要：【摘要】目的用黄芩苷干预观察胆管不全梗阻（PCBDL）大鼠肠黏膜损伤程度，通过检测肠黏膜核因子-κB（NF-κB）、NF-κBmRNA表达，探讨黄芩苷是否通过抑制NF-κB、NF-κBmRNA表达减轻胆管不全梗阻大鼠小肠黏膜损伤。方法随机数字表法将40只Wistar大鼠随机分为假手术组、PCBDL组、PCBDL＋黄芩苷处理1组（PCBDLl组）和PCBDL＋黄芩苷处理2组（PCBDL：组），每组10只。PCBDL、PCBDLl、PCBDL2组大鼠于近肝门处游离并不全结扎胆总管。PCBDL．组和PCBDL：组术后1d开始给予黄芩苷[80mg／（kg·d）]灌胃，其余组相同时间给予9g／L盐水1．5mL灌胃。观察大鼠回肠黏膜损伤并进行评分；应用免疫组织化学技术检测肠黏膜NF-κB的表达，原位杂交技术检测NF-κBmRNA的表达。结果1．与PCBDL组大鼠回肠黏膜损伤分级评分（3．2±0．5）比较，PCBDL，组（1．9±0．2）和PCBDL：组（1．5±0．3）大鼠回肠黏膜损伤分级评分明显降低（F=21．120，P〈0．01）；2．PCBDL组（0．155±0．012）回肠黏膜NF-κB蛋白的表达明显高于假手术组（0．066±0．006）、PCBDL1组（0．107±0．011）和PCBDL2组（0．098±0．010），差异有统计学意义（F=76．8，P〈0．01）；3．与假手术组、PCBDL，组和PCBDL2组比较，PCBDL组NF-κBmRNA表达的阳性率明显升高。结论本研究结果显示黄芩苷通过抑制肠黏膜上皮细胞NF-κB蛋白、NF-κBmRNA的表达，减轻大鼠胆管不全梗阻导致的肠黏膜损伤。Objective To investigate the effects of Baicalin on intestinal mucosal injury in rats with partial common bile duct ligation （PCBDL）. Methods Forty male Wistar rats were randomly divided into 4 groups equally： sham operation, PCBDL, PCBDL1 and PCBDL2. Rats in PCBDL, PCBDLI and PCBDL2 groups were subjected to partial common bile duct ligation. Baicalin [ 80 mg/（ kg · d） ] was fed in PCBDL1 group （for 2 weeks） and PCBDL2 group（ for 3 weeks） ,while for other groups ,9 g/L saline in the same volume was fed. Ileum mucosa were prepared for microscopic examination. The intestinal mucosal injury in rats was observed and scored. The level of NF-κB mRNA expression by Fluorescent in Situ Hybridization, and the level of NF-κB protein were determined by immunohistochemistry. Results 1. Compared with PCBDL group （3.2± 0. 5 ） , the pathological severity scores of intestinal mucosa significantly declined （F=21. 120,P 〈0. 01） in PCBDL1 group （1.9±0. 2） and PCBDL2 group （1.5 ±0. 3）.2. Compared with sham ope- ration group（0. 066 ± 0.006）, PCBDL1 group （ 0. 107 ± 0.011 ）, and PCBDL2 group （ 0.098±0. 010 ）, NF-κB expres- sion in PCBDL group （ O. 155 ± 0. 012 ） presented significantly up-regnlation （ F = 76. 8, P 〈 O. 01 ）. 3. Compared with sham operation, PCBDL1 group, and PCBDL2 group, the positive expression rates of NF-κB mRNA of intestinal mucosal epithelium in PCBDL group significantly increased. Conclusions It is suggested that Baicalin exert protective effects on the intestinal mucosal injury in rats with PCBDL,partially by inhibiting NF-κB mRNA, down-regulating NF-κB pro- tein expression of intestinal mucosal epithelial cells.

关 键 词：胆管不全梗阻 黄芩苷 肠黏膜上皮细胞 核因子-ΚB 核因子-ΚB mRNA 

分 类 号：R285.5[医药卫生—中药学]