澳蜡花‘Snow Flake’的组织培养试验研究  

An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake'

作　　者：娄利华[1] 曾静[1] 蒋海燕[1] 晏巧[1] 王正春[1] 

机构地区：[1]重庆市林业科学研究院,三峡库区森林生态保护与恢复重庆市市级重点实验室,重庆400036

出　　处：《四川林业科技》2014年第2期34-36,56,共4页Journal of Sichuan Forestry Science and Technology

基　　金：国家林业局948项目"新优木本花卉淘金彩梅及其相关高新技术的引进"(2011-4-36)

摘　　要：澳蜡花（Chamelauciumuncinatum）‘SnowFlake’作为新一代木本切花，具有较高的观花、观叶及经济价值。本试验通过对澳蜡花‘SnowFlake’组培技术的研究，为其进行工厂化繁育奠定基础。结果表明，用0．1％HgCl2进行外植体消毒最佳时间为7min；MSl＋6-BA1．0mg·L^-1＋IBA0．2mg·L^-1＋KT0．5mg·L^-1为最佳初代培养基；最适继代培养基为WPMl＋6-BA0．5mg·L^-1＋IBA0．2mg·L^-1＋GA32．5mg·L^-1，增殖系数4．2，增殖倍数3倍-6倍；最适生根培养基为I／2MS2＋IBA0．2mg·L^-1＋0．1mg·L^-1GGR。As a new generation of woody cut flowers, C. uncinatum ＇ Snow Flake＇ has a high ornamental and economic value. Based on the study of tissue culture of ＇ Snow Flake＇ , the aim of this experiment was to lay the foundation for Industrial production of ＇ Snow Flake＇ . The results showed that 7 minutes were the best time to sterilize explants by using 0.1% HgC12. MS1 ＋6 -BA1.0 mg·L-1 ＋ IBA0.2 mg.L-1 ＋ KT0. 5 mg-L-1 was the optimum primary culture medium,the best subcultuer medium was WPM1 ＋6 - BA 1.0 mg. L- 1 ＋ IBA 0. 2 mg. L- 1 ＋ GA3 1.5 mg＂ L- 1, multiplication coefficient was 4. 2, multiplication was 3 -6. The most suitable rooting medium was 1/2MS2 ＋ IBA0. 2 mg.L-1 ＋0. 1 mg-L-l GGR.

关 键 词：澳蜡花 组织培养 增殖 生根 

分 类 号：S685.99[农业科学—观赏园艺]