慢性铝中毒对大鼠海马 CA3 区神经元突触体素(p38)的影响  

The Morphometric Study Of The Effect Of Chronic Aluminium Toxicosis On p38-positive outgrowth In The Hippocampal CA3 Area Of Rats

作　　者：王继芬 康朝胜 臧贵勇[2] 孙宝飞[2] 

机构地区：[1]黔西南州人民医院神经内科,贵州兴义562400 [2]贵阳医学院解剖学教研室,贵州贵阳550004

出　　处：《中国卫生产业》2014年第11期20-20,22,共2页China Health Industry

摘　　要：目的通过突触体素（Synaptophysin，p38）的表达，探讨铝的神经毒性作用机理。方法40只4～5月龄sD大鼠，随机分为两组：铝中毒组和正常对照组，每组20只，雌雄各半。常规p38免疫组化染色，用细胞形态学计量方法测量p38阳性产物的平均光密度。结果铝中毒组大鼠海马CA3区神经元p38免疫组化染色阳性颗粒减少，阳性产物平均光密度降低，与正常对照组比较，差异有统计学意义（P〈0．01）。结论慢性铝中毒引起大鼠海马CA3区p38的减少。这种变化可能是铝的神经毒性作用机理之一。Objective To study the p38-positive outgrowth change of the hippocampal CA3 area of rats with chronic aluminium toxicosis.Methods 40 Sprague-Dawley rats of 4 -5 months age were randomly divided into two groups：The aluminum exposed group, aluminum were gaved for 3 months by digestive tract （ 100mgAlcl3/kg/d）,and normal control group, only equal distilled water were gaved, the p38-positive outgrowth in the hippocampal CA3 area were quantitatively analyzed with cell morphometric technique. Results The average optical density of p38 immunoreactivity positive structure were significantly decreased too in alu- minum exposed group than in the normal control group. Conclusion Aluminum can decrease the synthesis of p38 in the hip- pocampal CA3 area.It might be one of the mechanisms of aluminum neurotoxicity.

关 键 词：铝中毒 海马CA3区 突触体素 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]