静磁场对骨髓间充质干细胞增殖及骨向分化的影响  

Effects of Static Magnetic Field on Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells

作　　者：宋国丽[1] 周翠红[1] 张宇[1] 张小云[1] 

机构地区：[1]深圳大学生命科学学院,广东深圳市518060

出　　处：《中国康复理论与实践》2014年第4期322-326,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：深圳市科技研发资金基础研究计划项目(No.JC201005280537A)

摘　　要：目的：研究0.25 T、0.35 T、0.42 T静磁场持续或间歇曝磁对骨髓间充质干细胞（MSCs）增殖及骨向分化的影响。方法全骨髓贴壁筛选法分离MSCs，取第三代MSCs采用强度分别为0.25 T、0.35 T、0.42 T静磁场持续曝磁或间歇曝磁，CCK-8法检测各组细胞增殖活力，并观察0.35 T连续曝磁后MSCs碱性磷酸酶活性、骨钙素含量。结果细胞经过磁场处理后，与对照组相比，细胞增殖活力均降低，连续曝磁第7天效果最为显著（P＜0.001），而0.25 T、0.35 T间歇曝磁第2~8天持续表现出显著抑制效应（P＜0.001）。0.35 T持续曝磁后，MSCs碱性磷酸酶活性和骨钙素水平均增高（P＜0.05）。结论0.25 T、0.35 T、0.42 T静磁场连续曝磁或间歇曝磁均可抑制MSCs增殖，0.35 T连续曝磁能促进MSCs向成骨细胞方向分化。Objective To investigate the effect of exposure to 0.25 T, 0.35 T, 0.42 T static magnetic fields （SMF） on the proliferation and osteogenic differentiation of mesenchymal stem cells （MSCs）. Methods Primary bone marrow MSCs were obtained from Sprague-Dawley rats and screened by adhesive method. MSCs were exposed to 0.25 T, 0.35 T, 0.42 T SMF continuously and 24 h intermittently respectively. The cell proliferation activity was detected by Cell Counting Kit （CCK-8） assay. The osteogenic differentiation markers including alkaline phosphatase （ALP） activity and osteocalcin were analyzed after continuously exposure to 0.35 T SMF. Results Compared with the control group, the proliferation activity of SMF-treated cells significantly decreased, especially on the 7th day （P〈0.001） after continuous exposure, and on the 2nd to 8th day in 0.25 T, 0.35 T SMF intermittent exposure groups （P〈0.001）. Both the alkaline phosphatase activity and the lev-el of osteocalcin significantly increased in MSCs after continuous exposure to 0.35 T SMF （P〈0.05）. Conclusion Continuous or intermittent exposure to 0.25 T, 0.35 T and 0.42 T SMF could effectively inhibit the proliferation of MSCs. Continuous exposure to 0.35 T SMF could enhance the osteogenic differentiation of MSCs.

关 键 词：静磁场 骨髓间充质干细胞 增殖 骨向分化 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]