中国人类嗜T细胞病毒Ⅰ型汕头株前病毒外膜区核苷酸序列分析  

Nucleotide Sequences Analysis of Proviral Envelope Region of Shantou Strain of Human T lymphotropic Virus Type Ⅰ in China.

作　　者：庄文 肖泽斌[2] 曹凤[2] 贾桂芳[2] 季阳[2] 

机构地区：[1]汕头市中心血站,广东汕头515031 [2]中国医学科学院中国协和医科大学输血研究所

出　　处：《中国输血杂志》2001年第1期4-7,共4页Chinese Journal of Blood Transfusion

摘　　要：目的　了解来自中国汕头地区的人类嗜T细胞病毒Ⅰ型 (HTLV Ⅰ )外膜区基因特点。方法　设计 4条引物 ,用PCR扩增出HTLV Ⅰ汕头株 (命名为HTLV Ⅰ WHP)前病毒的外膜区基因 ,进行测序 ,并与来自世界各地的各型HTLV Ⅰ代表株进行比较、分析 ,构建进化树。结果　获得HTLV Ⅰ WHP前病毒的完整外膜区核苷酸序列 ,共 146 6bp。在推导的氨基酸序列中 ,无缺失、插入。进化树的分析结果表明 :HTLV Ⅰ WHP与来自日本、加勒比海的部分毒株最接近 ,同属于世界群 (cosmopolitangroup)a亚型。 结论　来自中国东南沿海地区以及日本、加勒比海的HTLVObjective To understand the gene characteristics of envelope region of human T lymphotropic virus type Ⅰ(HTLV Ⅰ)from Shantou region of China. Methods The gene of proviral envelope region of HTLV Ⅰ Shantou strain (named HTLV Ⅰ WHP)was amplified by PCR,using 4 designed primers,and then was sequenced,meanwhile it was compared with that of various HTLV Ⅰ representative strains from all around the world,and subsequently it was analyzed to construct its phylogenetic tree.Results The nucleotide sequence of complete envelope region of HTLV Ⅰ WHP provirus we have obtained was 1466 bp.There no deletion and insertion in the deducted amino acid sequence.The results of phylogenetic tree analysis showed that the HTLV Ⅰ WHP strain was most closed to some of the strains from Japan and Caribbean,and like the latter,belonged to the subtypea of cosmopolitan group.Conclusion The HTLV Ⅰ strains either from the coastal region of Southeast China or from Japan,Caribbean and so on have the common origin of evolution.

关 键 词：HTLV-I 外膜区基因 序列分析 基因分型 

分 类 号：R373[医药卫生—病原生物学]