股骨干骨折合并脑损伤后大鼠肺脏炎性介质与SP-A变化的实验研究  

Changes in Inflammatory Mediator and SP-A Following Femoral Shaft Fracture Combined with Traumatic Brain Injury in Rat Lungs

作　　者：刘钢[1,2] 宋耀宗[2] 于晨[2] 刘佳[2] 孙天胜[2] 

机构地区：[1]解放军医学院,北京100853 [2]北京军区总医院骨科,北京100700

出　　处：《医学综述》2014年第7期1280-1282,共3页Medical Recapitulate

基　　金：军队临床高新技术重大项目(2010jxjs007)

摘　　要：目的探讨大鼠股骨干骨折合并脑损伤后对肺脏表面活性蛋白A(SP-A)与炎性介质的影响。方法将108只SD雄性大鼠采用随机数字表法分为空白对照组(12只)、股骨干骨折组(32只)、脑损伤组(32只)、股骨干骨折合并脑损伤组(32只)。各组分别于1、6、12、24 h取材,取材部位为右肺下叶,应用Western Blot检测肺组织SP-A表达,酶联免疫吸附测定法检测肺组织中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)6、IL-10的水平。结果 6 h时骨折组、脑损伤组、骨折合并脑损伤组SP-A水平均升高,24 h时骨折合并脑损伤组SP-A降至对照组以下(P<0.05)。创伤后肺脏TNF-α、IL-6、IL-10均升高,骨折组、脑损伤组、骨折合并脑损伤组TNF-α、IL-6、IL-10水平均高于对照组(P<0.05),且骨折合并脑损伤组最高,与骨折组、脑损伤组比较差异统计学意义(P<0.05)。结论创伤后肺脏组织炎性介质及SP-A水平发生变化,与创伤程度密切相关。较轻的创伤会出现炎性介质升高,SP-A代偿性升高,严重创伤时SP-A失代偿性下降。Objective To study the influence of rat femoral fractures combined with traumatic brain injury on inflammatory mediator and SP-A in lungs. Methods Sprague-Dawley rats were randomly divided into 4 groups ： control group （ n = 12 ）, closed femoral fracture group （ n = 32 ）, traumatic brain injury group （n = 32）, closed femoral fracture combined with traumatic brain injury（ n = 32）. Got the part of the inferior lobe of right lung tissue at 1,6,12,24 h after modeling, Western Blot was employed to detect SP-A. Then levels of TNF-α,IL-6,IL-10 were dected with ELISA. Results The SP-A levels of three experiment groups increased compared with control group at 6 h. At 24 h SP-A of femoral fracture combined with traumatic brain injury group dropped below control group （ P 〈 0.05 ）. Trauma can improve the levels of TNF-α, IL-6, IL-10, three experiment groups had statistical significant difference compared with control group （ P 〈 0.05 ） and femoral fracture combined with traumatic brain injury group was the highest with statistically significant difference from the closed femoral fracture group and traumatic brand injury group（ P 〈 0.05 ）. Conclusion The level of inflammatory mediator and SP-A change after injury, and are closely related to the degree of trauma. Minor injury may increase the level of inflammatory mediator and SP-A, while trauma can decrease SP-A when uncompensated.

关 键 词：股骨干骨折 脑损伤 炎性介质 表面活性蛋白A 

分 类 号：R332[医药卫生—人体生理学]