蒲地兰颗粒的质量控制  

Quality Control of PuDiLan Granules

作　　者：王林凤[1] 魏国玲[1] 康毅[1] 

机构地区：[1]陕西中医学院附属医院,陕西咸阳712000

出　　处：《西部中医药》2014年第4期17-20,共4页Western Journal of Traditional Chinese Medicine

摘　　要：目的：建立蒲地兰颗粒的质量控制方法。方法：采用薄层色谱法对黄芩进行定性鉴别，采用HPLC法对黄芩中的黄芩苷进行含量测定。色谱柱为Thermo C18（250 mm×4.6 mm，5μm），流动相为甲醇-0.2%磷酸水溶液（47∶53），流速为1.0 mL/min，检测波长为280 nm。柱温为室温。结果：薄层色谱斑点清晰，阴性对照无干扰。黄芩苷在0.076～7.6μg范围内线性关系良好（r=0.9999），平均回收率为99.5%，RSD=0.71%（n=6）。结论：本研究建立的TLC和HPLC方法操作简便，结果准确，重复性好，专属性强，可用于该制剂的质量控制。Objective：To establish the method for quality control of PuDiLan granules. Methods： HuangQin （Scutellaria baicalensis） was identified qualitatively by TCL, the contents of baicalin in HuangQin were determined by HPLC. Chromatographic column：Thermo C18 （250 mm×4.6 mm, 5μm）, mobile phase：methanol-0.2%phosphoric acid solution （47：53）, flow rate was 1.0mL/min, detection wavelength：280nm, room temperature. Results：The spots of TLC were distinguished and clear. Baicalin showed better linear relationship in the range between 0.076 and 7.6μg （r=0.9999）, average recovery was 99.5%, RSD=0.71%（n=6）. Conclusion：TLC and HPLC method established in the study, simple, accurate, reproducible and specific, could be used to quality control of the preparation.

关 键 词：蒲地兰颗粒 质量控制 薄层色谱 高效液相色谱法 黄芩苷 

分 类 号：R284[医药卫生—中药学]