棘托竹荪菌盖蛋白提取及分析  

Study on Extraction and Analysis of Protein from Dictyophora echinovolvata Pileus

作　　者：曹萌[1,2] 林陈强[2] 林戎斌[2] 陈济琛[2] 邱宏端[1] 林新坚[2] 

机构地区：[1]福州大学生物科学与工程学院,福州350008 [2]福建省农业科学院土壤肥料研究所,福州350001

出　　处：《天然产物研究与开发》2014年第4期475-479,共5页Natural Product Research and Development

基　　金：福建省公益类科研院所项目(2011R1024-4);福建省科技重点项目(2012Y1003);福建省财政专项项目(2011-975)

摘　　要：首次以棘托竹荪菌盖为原料,通过单因素试验和正交试验,研究菌盖蛋白最佳提取工艺;用SDS-PAGE测定所提取蛋白的分子量分布。结果表明:提取时间和pH是影响竹荪菌盖蛋白提取的主要因数,最佳提取条件为pH8.0,提取时间2.5 h,提取温度55℃,提取率达64.07%,提取的竹荪菌盖主要蛋白分子量为16 kDa和38 kDa。棘托竹荪菌盖作为蛋白提取原料是可行的,以其为原料开发蛋白相关产品具有巨大的潜力和广阔的市场前景。Using Dictyophora echinovolvata pileus as the raw material,the optimal extraction process of protein was stud-ied by single factor and oahogonal experiments. The molecular weight distribution of extracted protein was analyzed by SDS-PAGE. The results indicated that the pH and extraction time were the most important factors to the extraction process. The highest extraction yield of 64.07% was obtained at pH 8.5,55 ℃ with 2.5 h of extraction. The major mo-lecular weights of extracted protein were 16 kDa and 38 kDa. In eonelusion,D, echinovolvata pileus is a suitable raw ma-terial for extracting proteins with great economic potential and market prospect.

关 键 词：竹荪菌盖 蛋白 提取 

分 类 号：Q946.1[生物学—植物学] R285[医药卫生—中药学]