罗仙子提取物对肝癌的体内外抑制作用研究  

Protective Effects of Chrysomya megacephala( Fab. ) Extracts on Hepatic Carcinoma in vitro and in vivo

作　　者：郑晓亮[1,2] 屠凌岚[1,2] 颜冬梅[1,2] 马臻[1] 彭小英[1] 

机构地区：[1]浙江省医学科学院药物研究所 [2]浙江省医学科学院分子医学中心,杭州310013

出　　处：《天然产物研究与开发》2014年第4期494-498,563,共6页Natural Product Research and Development

基　　金：浙江省科技厅省属科研院所专项项目(2007F10030);浙江省中医药科技计划项目(2006C042);浙江省医学重点学科群建设项目(XKQ-010-001)

摘　　要：本文观察中药罗仙子提取物在离体细胞水平以及整体动物模型中对肝癌的治疗作用。在罗仙子提取物干预人肝癌SMMC-7721细胞24 h后,采用MTT法检测细胞活率;采用光学显微镜、荧光显微镜、透射电镜观察细胞形态学改变,流式细胞仪检测细胞凋亡率;以Heps肝癌小鼠模型观察罗仙子提取物体内对肿瘤生长的抑制作用。研究发现不同浓度罗仙子提取物可抑制SMMC-7721细胞增殖同时诱导典型的细胞凋亡形态学变化,与对照组相比细胞凋亡率显著升高,且具有浓度依赖性;不同浓度罗仙子提取物可显著抑制Heps肝癌小鼠体内肿瘤生长。实验证明罗仙子提取物可显著抑制Heps肝癌小鼠体内肿瘤生长,该作用可能与罗仙子提取物抑制肝癌细胞增殖、诱导细胞凋亡有关。To observe the protective effects of Chrysomya megacephala （Fab.） extracts LXZ-1A on Hepatic carcinoma in vitro and in vivo. Cell viability of SMMC-7721 cells treated with LXZ-1A was measured by MTT assay. LXZ-1A-induced cell morphological changes were observed with the light microscope, fluorescence microscope and transmission electron microscope. Cell apoptosis rate was determined by flow cytometric analysis. The hepatoma model was established by sub- cutaneously inoculating Heps cells to ICR mice to evaluate the protective effect of LXZ-1A in vivo. The ICs0 （24 h） of LXZ-1A for inhibiting SMMC-7721 cell proliferation was 2.05 ＋ 0.11 mg/mL. The cell treated with LXZ-1A （0.3-1.0 mg/mL） showed typical morphological changes of apoptosis and the apeptotic rate was 28% to 50% for 24 h. The tumor growth in mice bearing Heps ceils was significandy inhibited by intraperitaneal injection of 0.16-1.0 g/kg LXZ-1A for 9 days. C. megacephala （ Fab. ） extracts LXZ-1A repressed the growth of xenograft tumours in Heps cell tumor-bearing mice by inhibiting cell proliferation and inducing cell apoptosis.

关 键 词：罗仙子提取物 肝癌 细胞凋亡 

分 类 号：R932[医药卫生—生药学]