D101大孔树脂富集纯化板蓝根木脂素活性部位的工艺研究  

Enrichment and Purification of Lignans from Radix Isatidis by D101 Macroporous Resin

作　　者：潘以琳[1] 李进[1] 李祥[1] 陈建伟[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《天然产物研究与开发》2014年第4期579-582,共4页Natural Product Research and Development

基　　金：江苏省产学研联合创新奖金项目(BY2009110);南京中医药大学"康缘中医药科技创新基金"项目(HZ0808KY);江苏高校优势学科建设工程资助项目(YSXK-2010)

摘　　要：本文以板蓝根总木脂素和Clemastanin B的含量为指标探讨D101大孔树脂纯化板蓝根木脂素活性部位的工艺。通过对树脂比容积、吸附容量、吸附流速、泄漏曲线、水洗终点、径高比、梯度解吸、解吸曲线等参数的考察,从而得出富集纯化板蓝根木脂素活性部位的最佳工艺条件为:上样量5 BV,径高比1∶3,吸附流速4 BV/h,1BV水洗,5 BV的50%乙醇洗脱。并证明了所选的大孔树脂纯化工艺稳定、可靠,有效成分损失少,杂质去除率高,值得在生产中推广应用。In this study,Radix Isatidis was purified by D101 macroporous resin. The purification conditions were opti- mized using the total active lignans and Clemastanin B as screening indexes. The influencing parameters including resin ratio of volume, adsorption capacity, adsorption velocity curve, leakage, washing end, ratio of diameter to height, gradient desorption and desorption curve were investigated. The optimum condition of enrichment and purification of active lignans from Radix Isatidis were as foUows：5 BV of the sample volume,1： 3 of the ratio of diameter to height,4 BV/h of the ad-sorption velocity, 1 BV of water elution and 5 BV of 50% ethanol elution. It was concluded that the purification process with D101 macroporous resin was stable,reliable,less loss of effective components, high impurity removal rate and worthy of popularization and application in large scale production.

关 键 词：板蓝根 大孔树脂 木脂素 Clemastanin B 

分 类 号：R284.2[医药卫生—中药学]