不规则抗体阳性结果分析及处理  

Analysis and treatment of the positive results in irregular antibody detection

作　　者：朱伟彦[1] 孔存权[1] 梁玉[1] 

机构地区：[1]河南省人民医院输血科,河南郑州450003

出　　处：《中国输血杂志》2014年第3期266-268,共3页Chinese Journal of Blood Transfusion

摘　　要：目的分析不规则抗体阳性结果,区分非特异性的自身抗体和有意义的同种抗体,指导临床用血。方法使用微柱凝胶卡进行不规则抗体实验,对阳性标本进行自身对照及抗体特异性检测,对自身对照阳性标本进行自身吸收放散处理后检测。结果不规则抗体阳性率0.36%,Rh血型系统抗体占65.71%,13例自身对照阳性经自身吸收放散后证实2例自身抗体、同种抗体同时存在,3例只存在同种抗体,8例非特异性抗体。结论不规则抗体阳性结果原因复杂,与患者疾病、输血史都有很大关系,自身对照阳性并非完全是非特异性抗体,需要进一步实验,保证输血安全。Objective To guide the clinical use of blood by analysing the positive results in irregular antibody detection and distinguishing the non-specific autoantibodies from significant alloantibodies. Methods The irregular antibody detection is performed by microcolumn gel card test. The positive samples are identified the specificity of antibodies based on the self-control test, and after that the absorption and elution test ;is carried out when the result of self-control is positive. Results The positive rate of irregular antibodies is 0.36% in which RH groups antibodies account for 65.71%. 13 cases are. positive in the self-control test. In the absorption and elution test,2 cases are confirmed the autoantibodies and alloantibodies existed at the same time,3 cases only contain isoantibody, and 8 cases contain non-specific antibodies. Conclusion The reasons for the production of the irregular antibodies are very complex ,There is a great relationship between the disease and the history of blood transfusion. The positive results of the self-control test is not entirely caused by the nnnspecific antibodies. So we must ensure the safety of blood transfusion by further experiments.

关 键 词：不规则抗体 抗体筛检 自身对照实验 RH血型 

分 类 号：R457.11[医药卫生—治疗学]