ALT异常献血者隐匿性丙型肝炎病毒感染筛查  

Detection of occult HCV infection in peripheral blood mononuclear cells among ALT abnormal blood donors

作　　者：林红[1] 朱绍汶[1] 陈显[1] 陈妍[1] 王静[1] 刘衍春[1] 孙俊[1] 黄成垠[1] 

机构地区：[1]江苏省血液中心,江苏南京210042

出　　处：《中国输血杂志》2014年第3期274-276,共3页Chinese Journal of Blood Transfusion

基　　金：江苏省卫生厅科技项目(No.H201225);江苏预防医学会科技项目(No.Y2012002)

摘　　要：目的了解献血者中隐匿性丙型肝炎病毒感染（OCI）情况；ALT是否对OCI献血者有筛查作用。方法从2012年9～12月血液常规筛查单纯ALT异常（ALT〉40U／L，核酸检测阴性）献血者血样中随机抽取其中113例（女性7名）作为实验组，并抽取同样数量的合格献血者做对照组。用Ficoll^R分离其外周血单核细胞（PBMCs），Trazol^R提取总RNA，逆转录一套式聚合酶链反应（RT-Nested PCR）扩增检测PBMCs中的HCVRNA。结果实验组中共检出9例（7．96％）PBMCs HCV RNA阳性，其中女性3例，男性6例；对照组未检出。结论ALT异常献血者中存在隐匿性丙型肝炎病毒感染，且女性高于男性，ALT对OCI献血者的筛查有一定意义。Objective To investigate the occult HCV infection in peripheral blood mononuclear cells （PBMCs） among ALT abnormal blood donors; and to evaluate if ALT screening has its Significance. Methods 113 blood samples of ALT ab-normal blood donors （ALT 〉 40 U/L, NAT negative） and the same number of qualified blood donors were selected randomly between September and December 2012. PBMCs were separated by Ficoll^R and total RNA were extracted by Trazol^R. HCV RNA in the PBMCs was amplified by RT nested-PCR. Results All healthy donor samples were tested negative for PBMCs HCV RNA;otherwise ,9/113 （7.96%）were positive for HCV RNA in PBMCs in ALT abnormal blood samples including 3 fe-males and 6 males which were identified as potential occult HCV infection. Conclusion It showed evidence that occult HCV infection might occur in abnormal ALT blood donors ,especially in female donors and ALT screening in blood donor is of certain significance.

关 键 词：无偿献血者 ALT异常 外周血单核细胞 隐匿性丙肝病毒感染 

分 类 号：R446[医药卫生—诊断学] R512.63[医药卫生—临床医学]