2010-2011年广州献血人群HIV-1流行病学分析  

Epidemiological analysis of HIV-1 Infection in Guangzhou blood donors from 2010 to 2011

作　　者：李玉笑[1] 肖韶英[1] 黄伯泉[1] 田也[1] 杜荣松[1] 汪传喜[1] 

机构地区：[1]广州血液中心检验科,广东广州510095

出　　处：《中国输血杂志》2014年第3期300-302,共3页Chinese Journal of Blood Transfusion

摘　　要：目的了解广州地区献血人群中艾滋病病毒1型（HIV-1）感染的流行病学情况。方法对广州地区581656例无偿献血者标本用2个不同厂家的ELISA法试剂同时检测抗-HIV，双试剂阳性者送广州市疾控中心用蛋白免疫印迹试验（WB）进行确认，从WB法阳性的献血者的血浆中提取核酸，用RT—PCR进行gag区基因扩增，对扩增产物进行测序和序列分析，并进行基因分型，用spss16．0软件对流行病学资料进行统计。结果ELISA法抗．HIV双试剂阳性133例（0．023％，133／581656），WB法检测120例HIV—1抗体阳性（0．021％，120／581656），不确定13例，120例WB法阳性的献血者中有6种亚型：CRF01-AE重组亚型51例；CRF07-BC重组亚型32例；CRF08．BC重组亚型15例；01B型16例；B’型6例，通过异性性传播组的阳性率高于其他途径传播组，男性组高于女性组，外地身价证的献血者高于广州市身份证者，首次献血者高于再次献血者，文化程度为初中及以下者高于其他组，年龄26～35岁组高于其他年龄组。结论本地区献血人群中HIV-1感染者以外地男性青壮年为主，主要通过性传播。Objective To evaluate the epidemiologieal status of HIV-1 infection in blood donors from Guang-zhou. Methods Totally 581 656 samples from blood donors were collected between Jan lst,2010 to Dec 31 th 2011. Anti-HIV of these samples were parallelly tested by two ELISA kits from different manufacturers. Samples with both positive ELISA results were confirmed by Western Blot （WB） in the Centers for Disease Control and Prevention in Gnangzhon. Based on the results of WB, RNA of HIV from positive samples was isolated and gag gene of HIV virus was amplified and sequenced by RT-PCR method to obtain the genotypes. Tile related statistic analysis was conducted using SPSS 16.0 software. Results An-ti-HIV was both positive in 133 out of 581,656 donors by using two different ELISA kits. （ 133/581,656,0. 023% ）. Among the 133 samples with positive ELISA results, 120 cases were positive for WB （120/581,656,0. 021% ）. Six sub-genotype of HIV-1 were identified in these 120 donors. CRF01-AE recombinant genotype were detected in 51 donors,CRF07-BC were de-tected in 32 donors,CRF08-BC were detected in 15 donors,OlB were detected in 16 donors,and B＇ were detected in 6 do-nors. The statistical analysis of anti-HIV distribution showed that heterosexual transmission is higher than the other routes of transmission. Male group is higher than female group. Ecdemic group is higher than local group. And first-time donors is high- er than fixed donors. Donors with junior high scbool degree or below have a higher risk than other groups, and donors age of 26-35 years have a higher risk than other age groups. Conclusion The majority of donors with HIV-1 infection in Guangzhou are young males from other places and the main way of transmission is across sexual transmition.

关 键 词：HIV-1 亚型 血液传播 献血者 

分 类 号：R193.3[医药卫生—卫生事业管理] R457.1[医药卫生—公共卫生与预防医学]