运动和低脂膳食对胰岛素抵抗大鼠肿瘤坏死因子α表达的影响  

Effects of exercise and low-fat diet on the TNF-α expression of insulin resistance in rats

作　　者：孙庆艳[1] 李苗苗[1] 李古州[1] 陆爱云[2] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]上海体育学院运动科学学院,上海200438

出　　处：《中国应用生理学杂志》2014年第2期180-183,共4页Chinese Journal of Applied Physiology

基　　金：安徽省高校省级优秀青年人才基金重点项目(2010SQRL027ZD);安徽师范大学培育基金项目(2011rcpy040);安徽师范大学博士科研启动基金;"安徽重要生物资源保护与利用研究"安徽省重点实验室科研基金

摘　　要：目的:研究长期运动和低脂膳食等生活方式的干预对胰岛素抵抗大鼠肿瘤坏死因子α(TNF-α)表达的影响。方法:130只大鼠随机分为:CON组,10只,低脂膳食喂养;HFD组,120只,高脂膳食喂养。喂养8周后,选取HFD组体重上游1/3的大鼠作为肥胖大鼠。进行一次口服葡萄糖耐量试验(OGTT)和胰岛素释放试验。整体胰岛素敏感性以葡萄糖-胰岛素指数评价。将肥胖的HFD大鼠再分为以下4组,每组10只,喂养8周:HFD-SED:高脂膳食;HFD-EX:高脂膳食,跑台运动;LFD-SED:低脂膳食;LFD-EX:低脂膳食,跑台运动。CON组继续低脂膳食喂养。再进行一次OGTT和胰岛素释放试验。以ELISA技术分析脂肪组织和比目鱼肌中TNF-α。结果:HFD组葡萄糖-胰岛素指数显著大于CON组。8周干预后,HFD-SED组葡萄糖-胰岛素指数显著大于CON组。3个干预组葡萄糖-胰岛素指数均显著小于HFD-SED组。HFD-SED组脂肪组织和比目鱼肌中TNF-α含量显著大于CON组,3个干预组均显著小于HFD-SED组。结论:运动和低脂膳食干预均能降低胰岛素抵抗大鼠组织中TNF-α的表达,从而缓解炎症应激,改善胰岛素抵抗。Objective： To investigate the effects of exercise （EX）, low-fat diet （LFD） and their combination intervention on the uunor necrosis factor-α（TNF-α）expression of insulin resistance in rots. Methods： One hundred and thirty male rats randomly assigned to 2 groups： Control（CON）-lO rats consuming a low-fat diet; HFD-120 mrs consuming a high-fat diet （HFD）. The dietary regimen was sustained for 8 wk, at which point the 40 HFD group rats gaining the most weight were referred to as the obese rats. Glucose tolerance was assessed by an oral glu cose tolerance test （OGTY）. During the procedure of OGTr, the blood was drawn for insulin assay （instdin release test）. The whole body in sulin resistance was assessed by glucose-insulin index. The obese HFD group rats were randomized into one of four intervention groups： I-IFD sedentary （HFD-SED）, HFD-exercise（HFD-EX）, low fat diet-SED （LFD-SED）, LFD-EX. Ex rats performed 8-wk exercise training on a motorized treadmill. The CON group had access to low-fat diet for another 8 wk. After 8 wk of exercise and low-fat diet intervention, the OGqT and insulin release test were performed again. To use ELISA technique for detecting TNF-α in soleus muscle and adipose tissue. Restflts： After being fed high-fat diet for 8 wk, glucose-insulin index in the HFD group were significantly greater than that in CON group （ P 〈 0.01）. After 8-week exercise and low-fat diet intervention, glucose-insulin index in HFD-SED group was significantly greater than that in CON group （ P 〈 0.01）. The index in three intervention groups was significantly less than that in HFD-SED group （ P 〈 0.01 ）. TNF-a content in adipose tissue and the soleus muscle for HFD-SED group was significantly greater than that in CON group （ P 〈 0.01）. Three intervention groups were signif icantly less than HFD-SED group （ P 〈 0.01 ）. Coneluslon： Exercise and low-fat diet interventions can decrease the TNF-α expression in in stalin resistance rat.

关 键 词：胰岛素抵抗 运动 低脂膳食 肿瘤坏死因子Α 大鼠 

分 类 号：G804.2[文化科学—运动人体科学]