小鼠经颈外静脉右心导管法测定心室压力的实验方法研究  

The study of catheterization to measure ventricular pressure in mice through venous cannula in external jugular vein

作　　者：刘盼盼[1] 颜双泉[1] 陈马云 邹丽珍[1] 黄晓颖[1] 王良兴[1] 

机构地区：[1]温州医科大学附属第一医院呼吸内科,浙江温州325000

出　　处：《中国应用生理学杂志》2014年第2期189-192,I0003,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(81270110);浙江省自然科学基金资助项目(LY13H010003)

摘　　要：目的:研究可行性高的测定小鼠右心室压力的实验方法。方法:通过自制PE导管,连接powerlab多通道生物信号记录系统,经颈外静脉插管,右心导管法测定小鼠右心室压力,并借以研究肺动脉压力变化。结果:用此方法对51只小鼠进行插管,46例成功进入心室并测压,成功率90.2%。其中33只正常小鼠中,成功30例,测得压力值:收缩压(23.4±5.7)mmHg,舒张压(3.7±2.6)mmHg,平均压(12.0±3.7)mmHg;18只肺动脉高压模型小鼠中,成功16例,测得压力值:收缩压:(32.2±2.8)mmHg,舒张压(3.8±2.0)mmHg,平均压(14.9±2.3)mmHg。共失败5例,经解剖发现2例进入下腔静脉,2例穿破心耳,1例穿破腋静脉进入胸壁。结论:使用自制的PE导管经颈外静脉插管,右心导管法测定右心室压具有成功率高、数据更准确、操作省时、方法易普及的优点。是一种较好的对小鼠进行右心室压力测定的方法。Objeelive： To study a feasible method of measuring right ventricular pressure by catheterization in mice. Melhods： Measuring the right ventricular pressure and the pulmonary artery pressure by homemade PE pipe through venous cannula in external jugular vein , using catheterization in mice with powerlab mulfimodal biometric signal recording system. Results： Forty-six out of 51 mice were experimented with this method smoothly and got a total success rate of 90.2%. Thirty of 33 normal mice and 16 of 18 mice with pulmonary arterial hypertension （PAH） were catheterized successftdly. The right ventricular pressure were as follow： systolic blood pressure： （23.4 ± 5.7） mmHg in normal group vs （32.2± 2.8） mmHg in mice with PAH, diastolic blood pressure： （3.7 ± 2.6） mmHg vs （3.8 ± 2.0）mmHg, mean pressure： （ 12.0 ± 3.7） mmHg vs （ 14.9 ± 2.3） mmHg. After autopsy for those 5 failed cases, we found that 2 cases were into the inferior vena cava, another 2 eases pierced the right auricle and the last one punctured the axillary vein into the chest wall. Condusion： Measuring the right ven- tricular pressure through venom cannula in extemal jugular vein with homemade PE pipe in mice gets not only a high success rate but also help to save time. Moreover, this method can be popularized easily. It is a good and feasible method for measuring fight ventricular pressure in mice.

关 键 词：右心导管 右心室压力 小鼠 

分 类 号：R332[医药卫生—人体生理学]